Isolation and characterization of a transposon mutant of Shewanella putrefaciens MR-1 deficient in fumarate reductase.
Isolation and characterization of a transposon mutant of Shewanella putrefaciens MR-1 deficient in fumarate reductase.
复制标题
富马酸还原酶缺陷的腐败希瓦氏菌 MR-1 转座子突变体的分离和表征。
DOI：
10.1046/j.1472-765x.1997.00196.x
复制
发表时间：
1997
影响因子：
2.4
通讯作者：
Myers,JM
中科院分区：
文献类型：
--
作者：
Myers,CR;Myers,JM
A transposon mutant, designated CMTn‐3, ofShewanella putrefaciensMR‐1 that was deficient in fumarate reduction was isolated and characterized. In contrast to the wild‐type, CMTn‐3 could not grow anaerobically with fumarate as the electron acceptor, and it lacked benzyl viologen‐linked fumarate reductase activity. Consistent with this, CMTn‐3 lacked a 65 kDac‐type cytochrome, which is the same size as the fumarate reductase enzyme. CMTn‐3 retained the wild‐type ability to use nitrate, iron(III), manganese(IV) and trimethylamineN‐oxide (TMAO) as terminal electron acceptors. The results indicate that the loss of the fumarate reductase enzyme does not affect other anaerobic electron transport systems in this bacterium.
