Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species
Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species
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DOI：
10.1093/nar/gkh562
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发表时间：
2004-04-01
影响因子：
14.9
通讯作者：
Fraser, CM
中科院分区：
文献类型：
--
作者：
Nelson, KE;Fouts, DE;Fraser, CM
在美国，与食源性疾病相关的三株单核细胞增生李斯特菌的基因组进行了全基因组比较分析。在单核细胞增多l.c oli菌株F2365（血清型4b，奶酪分离物）、F6854（血清型1/2a，法兰克福分离物）和H7858（血清型4b，肉类分离物）中分别鉴定出51、97和69个菌株特异性基因。83个基因局限于血清型1/2a， 51个基因局限于血清型4b。这些菌株和血清型特异性基因可能有助于观察到的致病性差异，以及生物体在各自环境中生存和生长的能力。血清型1/2a特异性基因包括一个编码鼠李糖生物合成途径的操纵子，该途径与胆酸生物合成有关，以及五个糖基转移酶和一个腺嘌呤特异性DNA甲基转移酶的操纵子。与菌株F2365相比，菌株H7858和菌株F6854基因组草图序列分别发现8603个和105 050个高质量单核苷酸多态性（snp）。全基因组比较分析表明，单胞增生l.s基因组基本上是同源的，大部分基因组差异包括噬菌体插入、转座元件和snp。
The genomes of three strains of Listeria monocytogenes that have been associated with food-borne illness in the USA were subjected to whole genome comparative analysis. A total of 51, 97 and 69 strain-specific genes were identified in L.monocytogenes strains F2365 (serotype 4b, cheese isolate), F6854 (serotype 1/2a, frankfurter isolate) and H7858 (serotype 4b, meat isolate), respectively. Eighty-three genes were restricted to serotype 1/2a and 51 to serotype 4b strains. These strain- and serotype-specific genes probably contribute to observed differences in pathogenicity, and the ability of the organisms to survive and grow in their respective environmental niches. The serotype 1/2a-specific genes include an operon that encodes the rhamnose biosynthetic pathway that is associated with teichoic acid biosynthesis, as well as operons for five glycosyl transferases and an adenine-specific DNA methyltransferase. A total of 8603 and 105 050 high quality single nucleotide polymorphisms (SNPs) were found on the draft genome sequences of strain H7858 and strain F6854, respectively, when compared with strain F2365. Whole genome comparative analyses revealed that the L.monocytogenes genomes are essentially syntenic, with the majority of genomic differences consisting of phage insertions, transposable elements and SNPs.