Timing and precision of rattlesnake spinal motoneurons are determined by the KV72/3 potassium channel
Timing and precision of rattlesnake spinal motoneurons are determined by the KV72/3 potassium channel
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DOI：
10.1016/j.cub.2023.11.062
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发表时间：
2024-01-22
期刊：
影响因子：
9.2
通讯作者：
Chagnaud，Boris P.
中科院分区：
文献类型：
--
作者：
Bothe，Maximilian S.;Kohl，Tobias;Chagnaud，Boris P.
新的运动行为的进化需要在控制肌肉活动的中央模式发生器（CPG）的修改。由于进化过程漫长，这些变化如何逐渐导致新的行为仍然是个谜。响尾蛇提供了一个独特的机会来研究运动CPG如何经过进化修饰以产生一种新的行为--在这种情况下，是声音信号。我们发现，响尾蛇的身体和尾部脊髓中的运动神经元（MN）具有根本不同的生理特性，这使得尾部的MN能够整合和传输CPG输出，以高时间精度控制超快肌肉。利用膜片钳电生理技术，我们证明了这些差异在运动和敲击MN主要是由KV 72/3钾通道。然而，尽管KV 72/3对运动和敲击MN生理产生了显著不同的影响，但单细胞RNA-seq出乎意料地没有揭示KV 72/3通道表达的任何差异。视频摘要
The evolution of novel motor behaviors requires modifications in the central pattern generators (CPGs) controlling muscle activity. How such changes gradually lead to novel behaviors remains enigmatic due to the long time course of evolution. Rattlesnakes provide a unique opportunity to investigate how a locomotor CPG was evolutionarily modified to generate a novel behavior—in this case, acoustic signaling. We show that motoneurons (MNs) in the body and tail spinal cord of rattlesnakes possess fundamentally different physiological characteristics, which allow MNs in the tail to integrate and transmit CPG output for controlling superfast muscles with high temporal precision. Using patch-clamp electrophysiology, we demonstrate that these differences in locomotor and rattle MNs are mainly determined by KV72/3potassium channels. However, although KV72/3exerted a significantly different influence on locomotor and rattle MN physiology, single-cell RNA-seq unexpectedly did not reveal any differences in KV72/3channels' expression.Video abstract