Comparative analysis of the renoprotective effects of pentoxifylline and vitamin E on streptozotocin-induced diabetes mellitus
Comparative analysis of the renoprotective effects of pentoxifylline and vitamin E on streptozotocin-induced diabetes mellitus
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DOI：
10.1081/jdi-200042728
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发表时间：
2005-01-01
期刊：
影响因子：
3
通讯作者：
Martínez-Morales, F
中科院分区：
文献类型：
--
作者：
Dávila-Esqueda, ME;Vertiz-Hernández, AA;Martínez-Morales, F
背景：糖尿病引起的氧化应激（OxS）在糖尿病肾病的发生发展中起重要作用。研究表明，抗氧化剂有利于减少它。维生素E已被证明是最能改善抗氧化状态的物质。最近，已提出戊茶碱（PTX）具有抗氧化性质。目的：本研究的目的是评估两种抗氧化剂在体内减少脂质过氧化和肾脏肥大的能力。方法：采用链脲佐菌素（STZ）诱导大鼠糖尿病模型。治疗组分为健康组（H）、糖尿病未治疗组（STZ）、PTX治疗组（STZ + PTX）和维生素E补充组（STZ + E）。在第8周，肾脏被删除;一个是匀浆定量脂质过氧化物水平（LPOS），另一个是用来研究形态学变化的电子显微镜（EM）。此外，定量血浆总抗氧化活性（TAA）。结果：PTX和维生素E组与STZ组相比，LPOS均降低。与STZ + E相比，PTX增加了TAA，STZ + E使其恢复至正常值。然而，两种治疗均将LPO/TAA比值降低至较低的基础水平;因此，在校正功能参数方面获得了相似的结果。STZ大鼠的结构变化包括肾小球膜增厚、足细胞扁平化以及内皮层开窗丢失。所有这些变化对给药大鼠的侵袭性较小。结论：维生素E和PTX具有潜在的治疗特性，可能有助于延缓糖尿病肾脏的恶化速度。
Background: Oxidative stress (OxS) induced by diabetes plays an important role in the development of diabetic nephropathy. Studies have shown that antioxidants are beneficial in its reduction. Vitamin E has been documented as providing the most improvement in the antioxidative status. Recently, pentoxifylline (PTX) has been proposed to have antioxidant properties. Aim: The aim of the study was to assess the ability of two antioxidants to reduce lipid peroxidation and renal hypertrophy in vivo. Methods: Diabetes was induced by streptozotocin (STZ) in rats. Treatment groups were divided as follows: healthy (H), diabetic without treatment (STZ), PTX treated group (STZ + PTX), and vitamin E supplemented (STZ + E) group. At 8 weeks, kidneys were removed; one was homogenized to quantify lipoperoxide levels (LPOS), and the other was used to study the morphological changes by electron microscopy (EM). Additionally, plasma total antioxidant activity (TAA) was quantified. Results: A reduction in LPOS was observed in both groups: PTX and vitamin E with regard to STZ group. PTX increased TAA compared to STZ + E, which restored it to its normal values. However, both treatments reduced the LPO/TAA ratio to lower basal levels; hence, similar results were obtained in terms of correcting functional parameters. Structural changes in STZ rats included a glomerular membrane thickening, podocyte flattening, as well as loss of fenestration in the endothelial layer. All these changes were less aggressive for treated rats. Conclusions: Vitamin E and PTX have potential therapeutic properties that may help to retard the rate of deterioration of diabetic kidneys.