A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicol
A novel biosensor based on competitive SERS immunoassay and magnetic separation for accurate and sensitive detection of chloramphenicol
复制标题
一种基于竞争性SERS免疫分析和磁分离的新型生物传感器，可准确、灵敏地检测氯霉素
DOI：
10.1016/j.bios.2016.01.064
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发表时间：
2016-06-15
影响因子：
12.6
通讯作者：
Dong, Ning
中科院分区：
文献类型：
--
作者：
Yang, Kang;Hu, Yongjun;Dong, Ning
氯霉素 (CAP) 的准确、灵敏检测对于公众健康和安全尤为重要。在这里，我们提出了一种基于竞争性表面增强拉曼散射 (SERS) 免疫测定和磁分离的残留 CAP 检测的新型传感器。在该纳米传感器中，功能化金纳米粒子 (AuNP) 被拉曼报告分子（例如 4,4'-联吡啶）标记。添加游离 CAP 后，游离 CAP 与上述 AuNP 之间引发竞争性免疫反应，以与 CAP 抗体修饰的磁性纳米颗粒 (MNP) 结合。在本传感器中，使用抗体缀合磁珠代替固体基质作为支撑材料和分离工具。借助磁铁，将混合物从上清液中去除以达到浓缩效果。这导致从上清液中获得的SERS信号强度发生明显变化。直接从上清液中收集SERS信号，这使得SERS测量更加稳定、可重复和可靠。所提出的基于 SERS 的磁性免疫传感器使我们能够在较宽的浓度范围 (1-1 x 10(4) pg/mL) 内以快速、选择性和灵敏 (1.0 pg/mL) 的方式检测 CAP。此外，这些结果表明，这种免疫传感器在实际水生环境中检测抗生素方面具有巨大的潜力，这对我们的生活至关重要。 (C) 2016 Elsevier B.V. 保留所有权利。
The accurate and sensitive detection of chloramphenicol (CAP) is particularly imperative to public health and safety. Here, we present a novel sensor for residual CAP detection based on competitive surface enhanced Raman scattering (SERS) immunoassay and magnetic separation. In this nanosensor, functionalized Au nanoparticles (AuNPs) were labeled with the Raman reporter molecule (e.g. 4,4'-dipyridyl). With the addition of free CAP, a competitive immune reaction was initiated between free CAP and above AuNPs for conjugating with CAP antibody-modified magnetic nanoparticles (MNPs). Instead of the solid substrate, the antibody conjugated-magnetic beads were used as supporting materials and separation tools in the present sensor. With the aid of a magnet, the mixture was removed from the supernatant for concentration effects. This caused obvious change of SERS signal intensity obtained from supernatant. The SERS signals were collected from the supernatant directly, which made the SERS measurements more stable, repeatable and reliable. The proposed SERS-based magnetic immunosensor allows us to detect CAP in a fast, selective and sensitive (1.0 pg/mL) manner over a wide concentration range (1-1 x 10(4) pg/mL). In addition, these results demonstrate that this immunosensor holds great potential for the detection of antibiotics in real aquatic environment, which is crucial to our life. (C) 2016 Elsevier B.V. All rights reserved.