CA1 pyramidal to basket and bistratified cell EPSPs: dual intracellular recordings in rat hippocampal slices
CA1 pyramidal to basket and bistratified cell EPSPs: dual intracellular recordings in rat hippocampal slices
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DOI：
10.1111/j.1469-7793.1998.201bu.x
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发表时间：
1998-02-15
影响因子：
5.5
通讯作者：
Thomson, AM
中科院分区：
文献类型：
--
作者：
Ali, AB;Deuchars, J;Thomson, AM
1。在成年大鼠海马切片的CAI区域的双重细胞内记录和突触连接的细胞的生物细胞填充填充物来研究在篮子（n = 7）中引起的兴奋性突触后电位（EPSP）（n = 7），并通过行动（n = 7）（n = 7）（n = 7）（n = 7）（n = 7）在同时记录的锥体细胞中激活的电势2。中间神经元可以根据其电生理特性细分为经典的快速尖峰，爆发，定期尖峰和快速尖峰细胞，并具有圆形的尖峰后磨后极化。但是，这些生理类别与形态类型无关。 EPSP未记录在常规的香料细胞中。3。在生殖细胞（范围为0.5-9 mV）中，平均EPSP幅度大于篮细胞（范围为0.15-3.6 mV），并且获得锥体细胞互助EPSP的概率也远高于生育细胞（1：7） ）比篮子细胞（1:22）。在繁殖细胞（0.7-2 ms）中，EPSP的增长时间为10-90％，其宽度为半振幅（3.9-11.2 ms）的宽度略长于篮细胞（上升时间，0.4-1.6 ms;半宽，2.2，2.2 -9.7 ms）.4。这些EPSP中的大多数（8个测试中的6个）随着突触后去极化的幅度和持续时间增加，尽管在两个（4个）篮细胞中，电压关系是常规的。5。在篮子（n = 7）中测试的所有EPSP和二级细胞（n = 5）均随重复性突触前发射而降低。在第一个EPSP的15毫秒内引起的第二个EPSP的平均幅度在第一个EPSP的平均幅度的34％至94％之间。短暂火车中的第三和第四个EPSP进一步沮丧。这种抑郁症与指示突触前基因座的透射率明显失败的发生率增加有关。
1. Dual intracellular recordings in the CAI region of adult rat hippocampal slices and biocytin filling of synaptically connected cells were used to study the excitatory postsynaptic potentials (EPSPs) elicited in basket (n = 7) and bistratified interneurones (n = 7) by action potentials activated in simultaneously recorded pyramidal cells.2. Interneurones could be subdivided according to their electrophysiological properties into classical fast spiking, burst firing, regular spiking and fast spiking cells with a rounded spike after-hyperpolarization. These physiological classes did not, however, correlate with morphological type. EPSPs were not recorded in regular spicing cells.3. Average EPSP amplitudes were larger in bistratified cells (range, 0.5-9 mV) than in basket cells (range, 0.15-3.6 mV) and the probability of obtaining a pyramidal cell-interneurone EPSP was also higher far the bistratified cells (1:7) than for the basket cells (1:22). EPSP 10-90% rise times in bistratified cells (0.7-2 ms) and their widths at half-amplitude (3.9-11.2 ms) were slightly longer than in basket cells (rise times, 0.4-1.6 ms; half-widths, 2.2-9.7 ms).4. The majority of these EPSPs (6 of 8 tested) increased in amplitude and duration with postsynaptic depolarization, although in two (of 4) basket cells the voltage relation was conventional.5. All EPSPs tested in both basket (n = 7) and bistratified cells (n = 5) decreased in amplitude with repetitive presynaptic firing. The average amplitudes of second EPSPs elicited within 15 ms of the first were between 34 and 94% of the average amplitude of the first EPSP. Third and fourth EPSPs in brief trains were further depressed. This depression was associated with an increase in the incidence of apparent failures of transmission indicating a presynaptic locus.