Tissue origins and interactions in the mammalian skull vault
Tissue origins and interactions in the mammalian skull vault
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DOI：
10.1006/dbio.2001.0487
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发表时间：
2002-01-01
影响因子：
2.7
通讯作者：
Morriss-Kay, GM
中科院分区：
文献类型：
--
作者：
Jiang, XB;Iseki, S;Morriss-Kay, GM
在哺乳动物的进化过程中，大脑半球的扩张伴随着颅骨穹窿的额骨和顶骨的扩张，以及冠状缝（额顶骨）和矢状缝（顶骨-顶骨）作为主要生长中心的展开。使用转基因小鼠与永久性神经嵴细胞谱系标记，Wnt 1-Cre/R26 R，我们表明，这两个缝线形成在神经嵴中胚层界面：额骨是神经嵴衍生和顶骨中胚层，与舌头的神经嵴之间的两个顶骨。通过使用X-gal染色的神经嵴迁移途径的详细分析，和中胚层跟踪DiI标记，我们表明，神经嵴-中胚层组织并列，后来形成冠状缝建立在E9.5作为尾侧边界的额鼻间充质。随着大脑半球的扩张，它们向尾部延伸，穿过真皮内的神经嵴-中胚层界面，携带着一层神经嵴细胞，形成它们的脑膜覆盖物。胚胎暴露于E10.0的视黄酸会降低脑膜神经嵴并抑制顶骨骨化，这表明中胚层骨的膜内骨化需要与神经嵴衍生的脑膜相互作用，而神经嵴衍生的额骨骨化是自主的。这些观察结果提供了新的观点头骨进化和人类遗传异常的头骨生长和骨化。(C)2001年，爱思唯尔科学。
During mammalian evolution, expansion of the cerebral hemispheres was accompanied by expansion of the frontal and parietal bones of the skull vault and deployment of the coronal (fronto-parietal) and sagittal (parietal-parietal) sutures as major growth centres. Using a transgenic mouse with a permanent neural crest cell lineage marker, Wnt1-Cre/R26R, we show that both sutures are formed at a neural crest-mesoderm interface: the frontal bones are neural crest-derived and the parietal bones mesodermal, with a tongue of neural crest between the two parietal bones. By detailed analysis of neural crest migration pathways using X-gal staining, and mesodermal tracing by DiI labelling, we show that the neural crest-mesodermal tissue juxtaposition that later forms the coronal suture is established at E9.5 as the caudal boundary of the frontonasal mesenchyme. As the cerebral hemispheres expand, they extend caudally, passing beneath the neural crest-mesodermal interface within the dermis, carrying with them a layer of neural crest cells that forms their meningeal covering. Exposure of embryos to retinoic acid at E10.0 reduces this meningeal neural crest and inhibits parietal ossification, suggesting that intramembranous ossification of this mesodermal bone requires interaction with neural crest-derived meninges, whereas ossification of the neural crest-derived frontal bone is autonomous. These observations provide new perspectives on skull evolution and on human genetic abnormalities of skull growth and ossification. (C) 2001 Elsevier Science.