Stimulated Raman photoacoustic imaging
Stimulated Raman photoacoustic imaging
复制标题
DOI：
10.1073/pnas.1012432107
复制
发表时间：
2010-11-23
影响因子：
11.1
通讯作者：
Scully, Marlan O.
中科院分区：
文献类型：
--
作者：
Yakovlev, Vladislav V.;Zhang, Hao F.;Scully, Marlan O.
在深层组织中实现具有高空间分辨率的无标记、分子特异性成像通常被认为是光学成像的巨大挑战。为了实现这一目标，必须克服组织中的显著光学散射，同时实现分子特异性而不诉诸于外部标记。我们证明了发展这样一种光学成像模式的可行性相结合的分子特定的受激拉曼激发与光声检测。通过采用两个超短激发激光脉冲，其在频率上由目标分子的振动频率分开，仅激发照射的组织体积中的目标分子的特定振动水平。该目标光学吸收产生超声波（称为受激拉曼光声波），使用传统的超声换能器检测该超声波以按照已建立的光声显微镜的设计形成图像。
Achieving label-free, molecular-specific imaging with high spatial resolution in deep tissue is often considered the grand challenge of optical imaging. To accomplish this goal, significant optical scattering in tissues has to be overcome while achieving molecular specificity without resorting to extrinsic labeling. We demonstrate the feasibility of developing such an optical imaging modality by combining the molecularly specific stimulated Raman excitation with the photoacoustic detection. By employing two ultrashort excitation laser pulses, separated in frequency by the vibrational frequency of a targeted molecule, only the specific vibrational level of the target molecules in the illuminated tissue volume is excited. This targeted optical absorption generates ultrasonic waves ( referred to as stimulated Raman photoacoustic waves) which are detected using a traditional ultrasonic transducer to form an image following the design of the established photoacoustic microscopy.