Phosphorylation status of Bβ subunit acts as a switch to regulate the function of phosphatase PP2A in ethylene-mediated root growth inhibition.
Phosphorylation status of Bβ subunit acts as a switch to regulate the function of phosphatase PP2A in ethylene-mediated root growth inhibition.
复制标题
Bβ亚基的磷酸化状态作为调控磷酸酶PP2A在乙烯介导的根生长抑制中的功能的开关。
DOI：
10.1111/nph.18467
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发表时间：
2022-12
期刊：
影响因子：
9.4
通讯作者：
Qiao, Hong
中科院分区：
文献类型：
--
作者：
Shao, Zhengyao;Zhao, Bo;Kotla, Prashanth;Burns, Jackson G.;Tran, Jaclyn;Ke, Meiyu;Chen, Xu;Browning, Karen S.;Qiao, Hong
关键词：
磷酸酶PP 2A全酶的不同亚基的各种组合和调节是其功能复杂性和重要性的基础。然而，PP 2A复合物响应外部或内部信号的分子机制仍然是未知的，特别是在拟南芥中。我们发现，PP 2A的Bβ磷酸化状态作为一个开关调节PP 2A的活性。在缺乏乙烯的情况下，磷酸化的Bβ导致PP 2A失活;底物EIR 1仍然被磷酸化，阻止EIR 1介导的生长素在表皮中的运输，导致正常的根生长。乙烯处理后，脱磷酸化的Bβ介导形成A2-C4-Bβ蛋白复合物，激活PP 2A，导致EIR 1脱磷酸化，促进生长素在伸长区表皮的运输，从而抑制根的生长。总之，我们的研究揭示了一种新的分子机制，通过Bβ亚基的去磷酸化开关PP 2A活性，使EIR 1去磷酸化，建立EIR 1介导的生长素在伸长区表皮中的运输，以响应乙烯对根生长的抑制。
The various combinations and regulations of different subunits of phosphatase PP2A holoenzymes underlie their functional complexity and importance. However, molecular mechanisms governing the assembly of PP2A complex in response to external or internal signals remain largely unknown, especially in Arabidopsis thaliana. We found that the phosphorylation status of Bβ of PP2A acts as a switch to regulate the activity of PP2A. In the absence of ethylene, phosphorylated Bβ leads to an inactivation of PP2A; the substrate EIR1 remains to be phosphorylated, preventing the EIR1‐mediated auxin transport in epidermis, leading to normal root growth. Upon ethylene treatment, the dephosphorylated Bβ mediates the formation of the A2–C4–Bβ protein complex to activate PP2A, resulting in the dephosphorylation of EIR1 to promote auxin transport in epidermis of elongation zone, leading to root growth inhibition. Altogether, our research revealed a novel molecular mechanism by which the dephosphorylation of Bβ subunit switches on PP2A activity to dephosphorylate EIR1 to establish EIR1‐mediated auxin transport in the epidermis in elongation zone for root growth inhibition in response to ethylene.
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影响因子：
2.9
作者：
Abas L;Luschnig C
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1912075117
发表时间：
2020-06-09
影响因子：
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