Localization of minor protein components of the head of bacteriophage T4
Localization of minor protein components of the head of bacteriophage T4
复制标题
噬菌体 T4 头部次要蛋白质成分的定位
DOI：
10.1128/jvi.24.1.121-134.1977
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发表时间：
1977
影响因子：
5.4
通讯作者：
M. Showe
中科院分区：
文献类型：
--
作者：
MULLER;Louise Onorato;M. Showe
噬菌体T4衣壳含有许多头部组装所需的次要蛋白质，但其在头部的详细功能和位置尚不清楚。我们已经发现，通过系统地改变提取条件，可以去除这些次要蛋白质中的一些，而主要衣壳结构基本上保持完整。残留衣壳的电子显微镜检查表明，基因20的产物的提取与区分一个顶点位置（推测为尾部附着位点）与其他顶点位置的插塞的丢失相关。基因24的产物的提取与衣壳的其他11个（非近端）顶点的损失相关。我们进一步表明，抗体P24特异性结合到非近端顶点的T4 preheads和T4 ESTA。我们的研究结果的基础上，我们认为，P20是位于或附近的尾部附着位点的衣壳，而P24形成的11个nonproximal顶点的preheads和P24形成的nonproximal顶点的成熟头部。
The bacteriophage T4 capsid contains a number of minor proteins that are required for head assembly but whose detailed function and position in the head are unknown. We have found that by systematically varying the conditions of extraction, some of these minor proteins can be removed while the main capsid structure is left substantially intact. Electron microscopic examination of the residual capsids showed that the extraction of the product of gene 20 is correlated with the loss of a plug that distinguishes one vertex position (presumably the tail attachment site) from the others. Extraction of the product of gene 24 is correlated with the loss of the other 11 (nonproximal) vertexes of the capsid. We further show that antibody to P24 binds specifically to the nonproximal vertexes of both T4 preheads and T4 phages. On the basis of our findings, we suggest that P20 is located at or near the tail attachment site of the capsid, whereas P24 forms the 11 nonproximal vertexes of preheads and P24 forms the nonproximal vertexes of the mature head.