Distribution of SV2C mRNA and protein expression in the mouse brain with a particular emphasis on the basal ganglia system
Distribution of SV2C mRNA and protein expression in the mouse brain with a particular emphasis on the basal ganglia system
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DOI：
10.1016/j.brainres.2010.09.063
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发表时间：
2011-01-07
期刊：
影响因子：
2.9
通讯作者：
Schiffmann, S. N.
中科院分区：
文献类型：
--
作者：
Dardou, D.;Dassesse, D.;Schiffmann, S. N.
突触囊泡2蛋白（Synaptic vesicle 2 protein，SV 2），SV 2A、SV 2B和SV 2C是位于所有神经元中突触囊泡表面的整合蛋白。SV 2蛋白似乎在突触囊泡胞吐和神经递质释放中起重要作用，但尚未完全理解。此外，SV 2似乎是肉毒杆菌神经毒素A的受体。在本研究中，使用单，双标记的荧光免疫组织化学和原位杂交，我们已经确定了脑模式的SV 2C mRNA和蛋白质的表达在小鼠。我们的研究结果表明，SV 2C蛋白的表达在一个小的子集的大脑区域，包括嗅球，嗅结节，丘脑核，尾壳核，腹侧苍白球，苍白球，黑质和腹侧被盖区。这些结果被证实通过原位杂交，除了苍白球和黑质网状部，其中没有标记被发现，表明在这些地区的SV 2C阳性纤维的纹状体投射神经元的终端。在纹状体中，我们发现，除了其存在于投射神经元中，SV 2C在一部分（约45%）胆碱能中间神经元中密集表达。此外，我们的数据还表明，SV 2C密集表达在大多数多巴胺能神经元的黑质pars延髓和腹侧被盖区（超过70%的多巴胺能神经元的分析是SV 2C阳性）。总之，我们的研究结果表明，SV 2C可能有助于调节神经递质释放和突触传递的基底神经节，包括胆碱能纹状体中间神经元和黑质纹状体/中脑边缘多巴胺神经元。(C)2010 Elsevier B. V.保留所有权利。
Synaptic vesicle 2 proteins (SV2), SV2A, SV2B and SV2C, are integral proteins localized on the surface of synaptic vesicles in all neurons. SV2 proteins appear to play an important, but not yet fully understood role in synaptic vesicle exocytosis and neurotransmitter release. Moreover, SV2 seems to be the receptor of the botulinum neurotoxin A. In the present study, using single and double-labeling fluorescent immunohistochemistry and in situ hybridization we have identified the brain pattern of SV2C mRNA and protein expression in mice. Our results indicated that SV2C protein was expressed in a small subset of brain regions including the olfactory bulb, olfactory tubercle, nucleus accumbens, caudate-putamen, ventral pallidum, globus pallidus, substantia nigra and the ventral tegmental area. These results were confirmed by means of in situ hybridization, except for the globus pallidus and the substantia nigra pars reticulata, in which no labeling was found, suggesting that SV2C-positive fibers in these areas are terminals of striatal projecting neurons. In the striatum, we found that, in addition to its presence in the projection neurons, SV2C was densely expressed in a fraction (around 45%) of cholinergic interneurons. In addition, our data also showed that SV2C was densely expressed in most dopaminergic neurons in the substantia nigra pars compacta and the ventral tegmental area (more than 70% of the dopaminergic neurons analyzed were SV2C-positive). Altogether, our results suggest that SV2C may contribute to the regulation of neurotransmitter release and synaptic transmission in the basal ganglia including cholinergic striatal interneurons and nigrostriatal/mesolimbic dopamine neurons. (C) 2010 Elsevier B.V. All rights reserved.