Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii.
Mutations at twelve independent loci result in absence of outer dynein arms in Chylamydomonas reinhardtii.
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DOI：
10.1083/jcb.107.6.2253
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发表时间：
1988-12
影响因子：
7.8
通讯作者：
Kamiya, R
中科院分区：
文献类型：
--
作者：
Kamiya, R
通过筛选慢泳表型，分离出35株缺失整个外动力蛋白臂的衣原体突变株。它们包含10个独立的遗传位点（odal-10），包括先前分离的突变体oda 38和pf 28。这10个位点与pf 13和pf 22不同，pf 13和pf 22是缺失外臂的非运动突变体的位点。这些结果表明，至少有12个基因负责外动力蛋白臂的组装。没有突变体缺乏外臂的部分结构，这表明缺乏单一组分导致整个外臂组装失败。两个不同的ODA菌株交配产生的临时双核体经常，但并不总是，在交配后2小时内恢复野生型运动。因此，外臂可以独立于鞭毛生长而被运输并附着到外双联体。
35 strains of Chlamydomonas mutant missing the entire outer dynein arm were isolated by screening slow-swimming phenotypes. They comprised 10 independent genetic loci (odal-10) including those of previously isolated mutants oda38 and pf28. The 10 loci were distinct from pf13 and pf22, loci for nonmotile mutants missing the outer arm. These results indicate that at least 12 genes are responsible for the assembly of the outer dynein arms. There were no mutants lacking partial structures of the outer arm, suggesting that lack of a single component results in failure of assembly of entire outer arms. Temporary dikaryons derived from mating of two different oda strains often, but not always, recovered the wild-type motility within 2 h of mating. Hence, outer arms can be transported and attached to the outer doublets independently of flagellar growth.