Luminescence studies of the intracellular distribution of a dinuclear gold(I) N-heterocyclic carbene complex
Luminescence studies of the intracellular distribution of a dinuclear gold(I) N-heterocyclic carbene complex
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DOI：
10.1002/anie.200601526
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发表时间：
2006-01-01
影响因子：
16.6
通讯作者：
Steer, James H.
中科院分区：
文献类型：
--
作者：
Barnard, Peter J.;Wedlock, Louise E.;Steer, James H.
2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Angew。化学。Int。[j] .中国生物医学工程学报。2006,45(5):566 - 570。近年来，n -杂环碳烯（NHC）配体作为潜在的靶向肿瘤细胞线粒体的抗肿瘤药物的发展，引起了人们的兴趣最近，我们报道了一个迷人的双核aui -碳配合物家族的合成和结构，例如，1和2这些复合物显示出显著的抗线粒体活性[3]，有趣的是，环烷配体框架可以很好地控制金原子之间的分子内距离。化合物1的Au··Au相互作用距离较短，为3.0485(3)，具有发光性，而化合物2的Au··Au相互作用距离较长，为3.7917(4)，不具有发光性从实验和理论的角度来看，与吸引的Au···Au（亲金）相互作用相关的发光引起了极大的兴趣，具有这种性质的配合物具有作为发光显示器件和传感器的潜力。[7]我们对靶向特定细胞器（线粒体）的兴趣使我们考虑利用荧光显微镜利用1的天然发光来确定其细胞内分布的可能性。然而，1 （lex 260 nm, lem 400 nm; ex=激发，em=发射）[4]的发光曲线不适合进行此类研究，因为高能量激发会导致细胞内其他分子的干扰。
5966 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Angew. Chem. Int. Ed. 2006, 45, 5966–5970 been interested in the development of AuI complexes of phosphine [2] and more recently N-heterocyclic carbene (NHC) ligands as potential antitumor agents that selectively target the mitochondria of cancer cells.[3] Recently, we reported the synthesis and structures of a family of fascinating dinuclear AuI–carbene complexes, for example, 1 and 2.[4] These complexes have demonstrated significant antimitochondrial activity [3] and, interestingly, the cyclophane ligand framework allows fine control over the intramolecular distance between the gold atoms. Compound 1 supports a short Au··· Au interaction of 3.0485 (3) and is luminescent, whereas compound 2, with a significantly longer Au··· Au distance (3.7917 (4)), is not.[4] The luminescence associated with attractive Au··· Au (aurophilic) interactions has been of great interest, both from experimental [5] and theoretical [6] points of view, and complexes possessing such properties offer potential as luminescent display devices and sensors.[7]Our interest in targeting specific cellular organelles (mitochondria) led us to consider the possibility of exploiting the native luminescence of 1 to determine its intracellular distribution by using fluorescence microscopy. However, the luminescence profile for 1 (lex 260 nm, lem 400 nm; ex= excitation, em= emission)[4] is unsuitable for such studies, as the high energy excitation would lead to interference from other molecules in the cell.