Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species
Epidermal bladder cells confer salinity stress tolerance in the halophyte quinoa and Atriplex species
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DOI：
10.1111/pce.12995
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发表时间：
2017-09-01
影响因子：
7.3
通讯作者：
Shabala, Sergey
中科院分区：
文献类型：
--
作者：
Kiani-Pouya, Ali;Roessner, Ute;Shabala, Sergey
已经假设表皮膀胱细胞（EBC）可以帮助盐植物应对盐水环境。但是，几乎没有直接支持证据。在这里，在盐水条件下种植藜麦植物5周。在盐度治疗前一天，比较了在刷子和非灌木丛中的生理，离子和代谢变化，通过使用柔软的化妆品刷以及生理，离子和代谢变化来轻轻去除所有叶子和叶柄的EBC。轻轻地去除EBC既没有引发伤口代谢，也没有影响控制植物的生理和生物化学，而是对盐生长的植物具有明显的作用，从而产生了对盐敏感的表型。在91个检测到的代谢产物中，有一半以上受盐度的显着影响。 EBC的去除急剧改变了这些代谢变化，报告了γ-氨基丁酸（GABA），脯氨酸，蔗糖和肌醇的最大差异，影响了跨细胞膜的离子转运（如电生理实验中所示）。这项工作为EBC在盐植物中的耐盐性中的作用提供了第一个直接证据，并将其归因于（1）EBC作为钠外部封存的盐转储的关键作用； （2）改善了叶叶叶叶和（3）EBC的K+保留率，作为已知调节植物离子关系的几种代谢产物的存储空间。
Epidermal bladder cells (EBCs) have been postulated to assist halophytes in coping with saline environments. However, little direct supporting evidence is available. Here, Chenopodium quinoa plants were grown under saline conditions for 5 weeks. One day prior to salinity treatment, EBCs from all leaves and petioles were gently removed by using a soft cosmetic brush and physiological, ionic and metabolic changes in brushed and non-brushed leaves were compared. Gentle removal of EBC neither initiated wound metabolism nor affected the physiology and biochemistry of control-grown plants but did have a pronounced effect on salt-grown plants, resulting in a salt-sensitive phenotype. Of 91 detected metabolites, more than half were significantly affected by salinity. Removal of EBC dramatically modified these metabolic changes, with the biggest differences reported for gamma-aminobutyric acid (GABA), proline, sucrose and inositol, affecting ion transport across cellular membranes (as shown in electrophysiological experiments). This work provides the first direct evidence for a role of EBC in salt tolerance in halophytes and attributes this to (1) a key role of EBC as a salt dump for external sequestration of sodium; (2) improved K+ retention in leaf mesophyll and (3) EBC as a storage space for several metabolites known to modulate plant ionic relations.