EXPRESSION OF THE C-FMS PROTO-ONCOGENE AND OF THE CYTOKINE, CSF-1, DURING MOUSE EMBRYOGENESIS
EXPRESSION OF THE C-FMS PROTO-ONCOGENE AND OF THE CYTOKINE, CSF-1, DURING MOUSE EMBRYOGENESIS
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DOI：
10.1016/0012-1606(89)90319-9
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发表时间：
1989-05-01
影响因子：
2.7
通讯作者：
ROSSANT, J
中科院分区：
文献类型：
--
作者：
REGENSTREIF, LJ;ROSSANT, J
c-fms基因编码集落刺激因子CSF-1的细胞表面受体。CSF-1最近已被证明在妊娠小鼠的母体子宫内膜中表达。应用原位杂交技术分析了小鼠原癌基因c-fms在小鼠胎盘发育过程中的个体发育和空间表达模式。在胚胎来源的组织中未检测到c-FMS表达，直到妊娠后（PC）9.5天，此时表达首次出现在壁滋养层巨细胞中。在整个妊娠期间，滋养层细胞中的表达持续处于高水平。在产后13.5天的成熟胎盘中，c-fms主要在海绵滋养层中表达，在迷路滋养层中表达较少。CSF-1在妊娠后8.5天在子宫上皮中首次检测到表达，这与妊娠后7.5天在发育中的卵柱周围的蜕膜细胞中c-fms的出现松散相关。这两个基因表达的时间过程和空间模式表明c-fms受体及其配体CSF-1在滋养层发育和分化中的功能作用。
The c-fms gene encodes the cell surface receptor of the colony-stimulating factor, CSF-1. CSF-1 has recently been shown to be expressed in the maternal uterine endometrium of pregnant mice. The ontogenetic and spatial patterns of expression of the murine proto-oncogene c-fms were analyzed in the developing mouse placenta by the technique of in situ hybridization. c-fms expression was not detected in fetally derived tissues until 9.5 days postcoitum (pc) when expression first appeared in the mural trophoblast giant cells. Expression persisted at high levels in trophoblast cells throughout gestation. In the mature placenta from 13.5 days pc on, c-fms was expressed chiefly in the spongiotrophoblast layer and, to a lesser extent, in the labyrinthine trophoblast. CSF-1 expression was first detectable in the uterine epithelium at 8.5 days pc which loosely correlated with the apperance at 7.5 days of c-fms in the decidual cells around the developing egg cylinder. The time course and spatial pattern of expression of tehse two genes suggest a functional role for the c-fms receptor and its ligand, CSF-1, in trophoblast development and differentiation.