Prospective characterization of neural stem cells by flow cytometry analysis using a combination of surface markers
Prospective characterization of neural stem cells by flow cytometry analysis using a combination of surface markers
复制标题
DOI：
10.1002/jnr.20442
复制
发表时间：
2005-05-15
影响因子：
4.2
通讯作者：
Nakahata, T
中科院分区：
文献类型：
--
作者：
Nagato, M;Heike, T;Nakahata, T
神经干细胞（NSCs）具有自我更新和多谱系分化特性，可以潜在地修复退化或受损的神经组织。在这里，我们通过荧光激活细胞分选（FACS），利用针对syndecan-1、Notch-1和整合素- β 1的抗体，从构成异质群体的神经球中富集NSCs，这些抗体被选为造血细胞或体细胞干细胞标记物的候选物。抗原阳性的细胞很容易启动神经球的形成，但缺乏这些标记的细胞则不那么容易。同时表达syndecan-1和Notch-1的双阳性细胞比单阳性细胞更能有效地形成神经球。分选细胞的后代在体外和体内均可分化为神经元和神经胶质细胞。这些抗体对于从小鼠胚胎14.5天的大脑中分离出有效形成神经球的细胞也很有用。相比之下，在Hoechst 33342染色的侧群细胞和主群细胞之间，神经球形成效率没有明显差异，尽管已知前者在各种组织中具有干细胞表型。这些结果表明syndecan-1、Notch-1和integrin- β 1作为NSC标记物的有效性。(c) 2005 Wiley-Liss, Inc。
Neural stem cells (NSCs) with self-renewal and multilineage differentiation properties can potentially repair degenerating or damaged neural tissue. Here, we have enriched NSCs from neurospheres, which make up a heterogeneous population, by fluorescence-activated cell sorting (FACS) with antibodies against syndecan-1, Notch-1, and integrin-beta 1, which were chosen as candidates for hematopoietic cell-or somatic stem cell-markers. Antigen-positive cells readily initiated neurosphere formation, but cells lacking these markers did so less readily. Doubly positive cells expressing both syndecan-1 and Notch-1 underwent neurosphere formation more efficiently than did singly positive cells. The progeny of sorted cells could differentiate into neurons and glial cells both in vitro and in vivo. These antibodies were also useful for isolating cells from the murine embryonic day 14.5 brain that efficiently formed neurospheres. In contrast, there was no distinct difference in neurosphere formation efficiency between Hoechst 33342-stained side population cells and main population cells, although the former are known to have a stem cell phenotype in various tissues. These results indicate the usefulness of syndecan-1, Notch-1, and integrin-beta 1 as NSC markers. (c) 2005 Wiley-Liss, Inc.