Chloroplast tubules visualized in transplastomic plants expressing green fluorescent protein.
Chloroplast tubules visualized in transplastomic plants expressing green fluorescent protein.
复制标题
在表达绿色荧光蛋白的转质体植物中可视化的叶绿体小管。
DOI：
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发表时间：
2000
影响因子：
4.9
通讯作者：
Y. Toyoshima
中科院分区：
文献类型：
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作者：
T. Shiina;K. Hayashi;N. Ishii;K. Morikawa;Y. Toyoshima
通过稳定质体转化将质体psbA启动子与绿色荧光蛋白基因（gfp）融合导入烟草叶绿体基因组。绿色荧光蛋白的合成积极和专门在叶绿体。第一次在这些transplastomic植物的叶绿体中观察到充满GFP但不含叶绿素的管状突起。有时，小管将叶绿体彼此连接，这表明内源蛋白质交换的可能性。然而，原生质体之间的融合的transplastomic和野生型植物表明，这种叶绿体连接可能是罕见的叶肉原生质体。
A fusion between the plastid psbA promoter and the green fluorescent protein gene (gfp) was introduced into the tobacco chloroplast genome by stable plastid transformation. GFP was synthesized actively and exclusively in the chloroplasts. Tubular projections filled with GFP but containing no chlorophyll were visualized for the first time in chloroplasts of these transplastomic plants. Occasionally, the tubules connect chloroplasts with each other, suggesting the possibility of the exchange of endogenous proteins. However, the fusion of protoplasts between the transplastomic and wild-type plants showed that such chloroplast connections might be rare in mesophyll protoplasts.
影响因子：
7.2
作者：
Kohler, RH;Zipfel, WR;Hanson, MR
通讯作者：
Hanson, MR
影响因子：
56.9
作者：
Kohler, RH;Cao, J;Hanson, MR
通讯作者：
Hanson, MR