A G1 cyclin is necessary for maintenance of filamentous growth in Candida albicans.
A G1 cyclin is necessary for maintenance of filamentous growth in Candida albicans.
复制标题
G1 细胞周期蛋白对于维持白色念珠菌的丝状生长是必需的。
DOI：
10.1128/mcb.19.6.4019
复制
发表时间：
1999
影响因子：
5.3
通讯作者：
Liu,H
中科院分区：
文献类型：
--
作者：
Loeb,JD;Sepulveda-Becerra,M;Hazan,I;Liu,H
白色念珠菌在不同的生长条件下会发生显著的形态学变化。这种从酵母形式转变为菌丝形式的能力是其致病性所必需的。传统的遗传学方法难以处理的数据阻碍了对这种发育转换的分子机制的研究。我们的方法是使用遗传上更易处理的酵母Saccharomycesgallaeto产生的线索，分子控制的发酵，为进一步研究在念珠菌。G1细胞周期蛋白Cln 1和Cln 2参与了酿酒酵母形态发生的调控。我们证明了C.白念珠菌CLN 1（CaCLN 1）具有与S.啤酒。研究G1细胞周期蛋白是否同样参与了C. Cacln 1/Cacln 1细胞的细胞周期进程比野生型细胞慢。Cacln 1/Cacln 1突变体在几种固体培养基上的菌丝集落形成也有缺陷。此外，虽然突变菌株在几种菌丝诱导条件下形成芽管，但它们不能在合成的菌丝诱导液体培养基中维持菌丝生长模式，并且在该培养基中缺乏菌丝特异性基因的表达。我们的研究结果表明，CaCln 1可能协调调节菌丝发育的信号转导途径，以响应各种环境线索。
Candida albicansundergoes a dramatic morphological transition in response to various growth conditions. This ability to switch from a yeast form to a hyphal form is required for its pathogenicity. The intractability ofCandidato traditional genetic approaches has hampered the study of the molecular mechanism governing this developmental switch. Our approach is to use the more genetically tractable yeastSaccharomyces cerevisiaeto yield clues about the molecular control of filamentation for further studies inCandida. G1cyclins Cln1 and Cln2 have been implicated in the control of morphogenesis inS. cerevisiae. We show thatC. albicans CLN1(CaCLN1) has the same cell cycle-specific expression pattern asCLN1andCLN2ofS. cerevisiae. To investigate whether G1cyclins are similarly involved in the regulation of cell morphogenesis during the yeast-to-hypha transition ofC. albicans, we mutatedCaCLN1.Cacln1/Cacln1cells were found to be slower than wild-type cells in cell cycle progression. TheCacln1/Cacln1mutants were also defective in hyphal colony formation on several solid media. Furthermore, while mutant strains developed germ tubes under several hypha-inducing conditions, they were unable to maintain the hyphal growth mode in a synthetic hypha-inducing liquid medium and were deficient in the expression of hypha-specific genes in this medium. Our results suggest that CaCln1 may coordinately regulate hyphal development with signal transduction pathways in response to various environmental cues.