Proliferation and differentiation of adipose-derived stem cells on naturally derived scaffolds
Proliferation and differentiation of adipose-derived stem cells on naturally derived scaffolds
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DOI：
10.1016/j.biomaterials.2007.12.028
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Woodhouse, Kimberly A.
中科院分区：
文献类型：
--
作者：
Flynn, Lauren E.;Prestwich, Glenn D.;Woodhouse, Kimberly A.
重建整形外科需要一种组织工程替代物，促进皮下脂肪组织层的大量再生。为了实现这一目标，我们描述了在体外培养的原代人脂肪来源的干细胞（ASC）接种到胎盘脱细胞基质（PDM）和交联透明质酸（XLHA）支架。具体而言，我们评估了PDM、XLHA和PDM与XLHA组合支架中的细胞增殖和成脂分化。在诱导分化之前测定细胞增殖、活力和葡萄糖消耗。通过使用终点和真实的时间逆转录聚合酶链反应（RT-PCR）的基因表达分析研究每个支架内的脂肪生成。结果表明，细胞粘附PDM支架促进增殖和活力，而分化增强时，细胞被封装在非粘性XLHA凝胶。(C)2008爱思唯尔有限公司保留所有权利。
A tissue-engineered substitute that facilitates large-volume regeneration of the subcutaneous adipose tissue layer is needed for reconstructive plastic surgery. Towards this goal, we describe the in vitro culture of primary human adipose-derived stem cells (ASC) seeded into placental decellular matrix (PDM) and cross-linked hyaluronan (XLHA) scaffolds. Specifically, we evaluated cellular proliferation and adipogenic differentiation in the PDM, XLHA, and PDM combined with XLHA scaffolds. Cellular proliferation, viability, and glucose consumption were determined prior to the induction of differentiation. Adipogenesis within each of the scaffolds was investigated through gene expression analysis using end point and real time reverse transcriptase polymerase chain reaction (RT-PCR). The results indicate that the cell-adhesive PDM scaffolds facilitated proliferation and viability, while differentiation was augmented when the cells were encapsulated in the non-adhesive XLHA gels. (C) 2008 Elsevier Ltd. All rights reserved.