The E2 "gene" of bovine papillomavirus encodes an enhancer-binding protein.
The E2 "gene" of bovine papillomavirus encodes an enhancer-binding protein.
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牛乳头瘤病毒的E2“基因”编码增强子结合蛋白。
DOI：
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发表时间：
1987
影响因子：
11.1
通讯作者：
D. Bastia
中科院分区：
文献类型：
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作者：
C. Moskaluk;D. Bastia
将牛乳头瘤病毒1型E2早期开放阅读框（推测为基因）与载体pJG 200的胶原-β-半乳糖苷酶编码区框内融合，并在大肠杆菌中表达和部分纯化。杂交蛋白特异性结合牛乳头瘤病毒的增强子区域的几个位点。一个这样的网站的DNA酶I-切割保护分析揭示了受保护的序列。将受保护的序列与也对蛋白质具有亲和力的其余DNA序列进行比较，揭示了具有基序AATTGGCGGNNCG的共有序列，其中N是任何核苷酸。保护区还包括具有2重旋转对称性的序列-ATCGGTG/CACCGAT。
The E2 early open reading frame (presumably gene) of bovine papillomavirus-1 was fused in frame with the collagen-beta-galactosidase-encoding region of the vector pJG200 and was expressed in and partially purified from Escherichia coli. The hybrid protein specifically bound to the enhancer region of bovine papillomavirus at several sites. DNase I-cleavage protection analysis of one such site revealed the protected sequence. A comparison of the protected sequence with the remainder of the DNA sequences that also have affinity for the protein revealed a consensus sequence having the motif AATTGGCGGNNCG, in which N is any nucleotide. The protected region also includes a sequence with 2-fold rotational symmetry--ATCGGTG/CACCGAT.