Kinesin superfamily proteins (KIFs) in the mouse transcriptome
Kinesin superfamily proteins (KIFs) in the mouse transcriptome
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DOI：
10.1101/gr.984503
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发表时间：
2003-06-01
期刊：
影响因子：
7
通讯作者：
Hirokawa, N
中科院分区：
文献类型：
--
作者：
Miki, H;Setou, M;Hirokawa, N
在几乎所有基因和蛋白质都已知的后基因组时代，一项重要任务是对重要基因类别的表达进行全面分析，例如细胞内运输所需的基因。我们报告了对驱动蛋白超家族的全面分析，这是第一个也是唯一一个其成分已在计算机和 cDNA、mRNA 水平上完全鉴定和确认的大型蛋白质家族。在 FANTOM2 中，我们发现了来自 33 个驱动蛋白超家族蛋白 (KIF) 基因位点的 90 个克隆。根据序列状态、来源文库、检测方法和可变剪接对克隆进行分析。超过一半的代表性转录单位（TU）是全长的。 FANTOM2 库还包含以前未报道的新颖剪接变体。我们使用该数据集比较和评估了各种蛋白质分类工具和蛋白质搜索方法。该报告为未来沿着微管的细胞内转运研究奠定了基础，并证明了细胞内转运蛋白转录本作为转录组一部分的重要性。
In the post genomic era where virtually all the genes and the proteins are known, an important task is to provide a comprehensive analysis of the expression of important classes of genes, such as those that are required for intracellular transport. We report the comprehensive analysis of the Kinesin Superfamily, which is the first and only large protein family whose constituents have been completely identified and confirmed in silico and at the cDNA, mRNA level. In FANTOM2, we have found 90 clones from 33 Kinesin Superfamily Protein (KIF) gene loci. The clones were analyzed in reference to sequence state, library of origin, detection methods, and alternative splicing. More than half of the representative transcriptional units (TU) were full length. The FANTOM2 library also contains novel splice variants previously unreported. We have compared and evaluated various protein classification tools and protein search methods using this data set. This report provides a foundation for future research of the intracellular transport along microtubules and proves the significance of intracellular transport protein transcripts as part of the transcriptome.