Altered brain lipid composition in cyclooxygenase-2 knockout mouse
Altered brain lipid composition in cyclooxygenase-2 knockout mouse
复制标题
DOI：
10.1194/jlr.m600400-jlr200
复制
发表时间：
2007-04-01
影响因子：
6.5
通讯作者：
Basselin, Mireille
中科院分区：
文献类型：
--
作者：
Ma, Kaizong;Langenbach, Robert;Basselin, Mireille
环氧合酶（考克斯）-2在脑花生四烯酸（20：4 n-6）代谢中起重要作用，在神经炎症和兴奋毒性动物模型中表达上调。我们的假设是，与野生型（考克斯-2（+/+））小鼠相比，考克斯-2敲除小鼠（考克斯-2（-/-））的脑脂质组成将发生改变，反映了考克斯-2在脑脂质代谢中的重要作用。在来自考克斯-2（-/-）和考克斯-2（+/+）小鼠的高能微波脑中测量不同脂质的浓度。与考克斯-2（+/+）小鼠脑相比，考克斯-2（-/-）小鼠脑的磷脂酰丝氨酸（PtdSer）在统计学上显著增加15%，三酰甘油和胆固醇浓度以及胆固醇-磷脂比分别显著降低37%、27%和32%。PtdSer中棕榈酸的标准化浓度（16：0）增加，未酯化花生酸的脑浓度（20：0）也增加。终生缺乏考克斯-2会导致脑脂质组成的多种变化。这些变化可能与报告的脂肪酸动力学变化以及考克斯-2（-/-）小鼠对神经炎症和兴奋性毒性的抵抗力有关。
Cyclooxygenase (COX)-2 plays an important role in brain arachidonic acid (20:4n-6) metabolism, and its expression is upregulated in animal models of neuroinflammation and excitotoxicity. Our hypothesis was that brain lipid composition would be altered in COX-2 knockout (COX-2(-/-)) compared with wild-type (COX-2(+/+)) mice, reflecting the important role of COX-2 in brain lipid metabolism. Concentrations of different lipids were measured in high-energy microwaved brain from COX-2(-/-) and COX-2(+/+) mice. Compared with the COX-2(+/+) mouse brain, the brain of the COX-2(-/-) mouse had a statistically significant 15% increase in phosphatidylserine (PtdSer) and significant 37, 27, and 32% reductions in triacylglycerol and cholesterol concentrations and in the cholesterol-to-phospholipid ratio, respectively. ne normalized concentration of palmitic acid (16:0) was increased in PtdSer, as was the brain concentration of unesterified arachidic acid (20:0). A lifetime absence of COX-2 produces multiple changes in brain lipid composition. These changes may be related to reported changes in fatty acid kinetics and in resistance to neuroinflammation and excitotoxicity in the COX-2(-/-) mouse.,