A dielectric barrier discharge ionization based interface for online coupling surface plasmon resonance with mass spectrometry.
A dielectric barrier discharge ionization based interface for online coupling surface plasmon resonance with mass spectrometry.
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DOI：
10.1039/c6an00561f
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
Yiding Zhang;Shuting Xu;Luhong Wen;Yu Bai;Li Niu;D. Song;Huwei Liu
中科院分区：
文献类型：
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作者：
Yiding Zhang;Shuting Xu;Luhong Wen;Yu Bai;Li Niu;D. Song;Huwei Liu
表面等离子体共振（SPR）与质谱（MS）的在线结合有利于在高通量下同时准确地获得分子相互作用数据及其结构信息。在这项工作中，一种新的SPR-MS接口的开发使用介质阻挡放电电离（DBDI）源。DBDI源被放置在MS入口的前面，产生电离等离子体射流。在DBDI源出口和MS入口之间设置喷雾头，雾化SPR样品溶液。利用该接口，样品可以先进行SPR研究，然后喷雾电离，最后进行MS分析。通过对含盐溶液中溶解的小分子药物模型样品的分析，观察到SPR和MS信号的一致性变化，证明了该接口的实用性。与我们以前开发的基于真实的时间直接分析（DART）的SPR-MS接口相比，该接口对非挥发性盐的耐受性更高，对不同样品的电离能力也不同。这些结果表明，该接口可以找到进一步利用SPR-MS研究，特别是在生理条件下需要。
The online combination of surface plasmon resonance (SPR) with mass spectrometry (MS) could be beneficial for accurately acquiring molecular interaction data simultaneously with their structural information at high throughputs. In this work, a novel SPR-MS interface was developed using a dielectric barrier discharge ionization (DBDI) source. The DBDI source was placed in front of the MS inlet, generating an ionization plasma jet. A spray tip was set between the DBDI source outlet and the MS inlet, nebulizing the SPR sample solution. Using this interface, samples could first be studied by SPR, then sprayed and ionized, finally analyzed by MS. By analyzing model samples containing small-molecule drugs dissolved in salt containing solutions, the practicability of this SPR-DBDI-MS interface was proved, observing the consistent change of SPR and MS signals. Compared with our previously developed direct analysis in real time (DART) based SPR-MS interface, this new interface exhibited a higher and better tolerance to non-volatile salts, and different ionization capabilities for various samples. These results indicated that the interface could find further utilization in SPR-MS studies especially when physiological conditions were needed.