Searching Fast for a Target on DNA without Falling to Traps
Searching Fast for a Target on DNA without Falling to Traps
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DOI：
10.1103/physrevlett.103.138102
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发表时间：
2009-09-25
影响因子：
8.6
通讯作者：
Voituriez, R.
中科院分区：
文献类型：
--
作者：
Benichou, O.;Kafri, Y.;Voituriez, R.
基因组表达关键取决于调节蛋白在几秒钟内定位 DNA 上特定靶位点的能力，以及这些蛋白和 DNA 之间长寿命（许多分钟）复合物的形成。平衡实验表明调节蛋白确实与其靶位点紧密结合。然而，他们还发现与其他非特异性位点的强结合，这些非特异性位点充当陷阱，可以大大增加定位目标所需的时间。这就造成了速度和稳定性要求之间的冲突。在这里，我们提出了一个简单的机制来解决这个长期存在的悖论。
Genomic expression depends critically on both the ability of regulatory proteins to locate specific target sites on DNA within seconds and on the formation of long-lived ( many minutes) complexes between these proteins and the DNA. Equilibrium experiments show that indeed regulatory proteins bind tightly to their target site. However, they also find strong binding to other nonspecific sites which act as traps that can dramatically increase the time needed to locate the target. This gives rise to a conflict between the speed and stability requirements. Here we suggest a simple mechanism which can resolve this long-standing paradox.