Preproglucagon (PPG) neurons innervate neurochemically identified autonomic neurons in the mouse brainstem.
Preproglucagon (PPG) neurons innervate neurochemically identified autonomic neurons in the mouse brainstem.
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DOI：
10.1016/j.neuroscience.2012.09.071
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发表时间：
2013-01-15
期刊：
影响因子：
3.3
通讯作者：
Trapp, S.
中科院分区：
文献类型：
--
作者：
Llewellyn-Smith, I. J.;Gnanamanickam, G. J. E.;Reimann, F.;Gribble, F. M.;Trapp, S.
关键词：
胰高血糖素前体（PPG）神经元产生胰高血糖素样肽-1 (GLP-1)，主要发生在孤束核（NTS）。GLP-1影响多种中枢自主神经回路，包括那些控制心血管系统、产热和最显著的能量平衡的回路。我们对在PPG启动子控制下表达YFP的转基因小鼠的免疫组织化学研究表明，PPG神经元广泛投射到中枢自主神经区域，包括脑干核。功能研究强调了GLP-1厌食作用的后脑受体的重要性。在这项研究中，我们评估了转基因YFP- ppg小鼠神经化学鉴定的脑干神经元的YFP神经支配。YFP与胆碱乙酰转移酶（ChAT）、酪氨酸羟化酶（TH）和/或5-羟色胺（5-HT）的免疫反应性通过使用黑色（YFP）、棕色和蓝灰色反应产物标记的二色或三色免疫过氧化物酶进行可视化。在迷走神经背侧运动核（DMV）中，细小的yfp免疫反应性轴突的末端与一小部分chat阳性和罕见的th阳性/ chat阳性运动神经元紧密相连，主要位于AP的腹侧。yfp免疫反应性神经支配几乎不存在于紧密和松散的模糊性核结构中。在NTS中，一些th免疫反应神经元与含有yfp的轴突紧密相连。在腹外侧髓质的A1/C1柱中，th阳性神经元的紧密重合更为常见，尤其是在柱的尾部部分。单个yfp免疫反应性轴突通常在单个ChAT-或th阳性神经元上提供1-3个密切关联。5 -羟色胺免疫反应神经元受神经支配最为严重，大部分苍白斑神经元、隐斑神经元和副锥体神经元受到yfp免疫反应轴突大变异的几个密切关联。这些结果表明，GLP-1神经元支配着不同的脑干自主神经元群。其中包括与心血管控制相关区域的迷走神经传出神经元和儿茶酚胺神经元。我们的数据还表明，GLP-1神经元和5-HT神经元之间存在突触连接，其中一些可能有助于食欲的调节。
Preproglucagon (PPG) neurons produce glucagon-like peptide-1 (GLP-1) and occur primarily in the nucleus tractus solitarius (NTS). GLP-1 affects a variety of central autonomic circuits, including those controlling the cardiovascular system, thermogenesis, and most notably energy balance. Our immunohistochemical studies in transgenic mice expressing YFP under the control of the PPG promoter showed that PPG neurons project widely to central autonomic regions, including brainstem nuclei. Functional studies have highlighted the importance of hindbrain receptors for the anorexic effects of GLP-1. In this study, we assessed YFP innervation of neurochemically-identified brainstem neurons in transgenic YFP-PPG mice. Immunoreactivity for YFP plus choline acetyltransferase (ChAT), tyrosine hydroxylase (TH) and/or serotonin (5-HT) were visualised with two- or three-colour immunoperoxidase labelling using black (YFP), brown and bluegrey reaction products. In the dorsal motor nucleus of the vagus (DMV), terminals from fine YFP-immunoreactive axons closely apposed a small proportion of ChAT-positive and rare TH-positive/ChAT-positive motor neurons, mostly ventral to AP. YFP-immunoreactive innervation was virtually absent from the compact and loose formations of the nucleus ambiguus. In the NTS, some TH-immunoreactive neurons were closely apposed by YFP-containing axons. In the A1/C1 column in the ventrolateral medulla, close appositions on TH-positive neurons were more common, particularly in the caudal portion of the column. A single YFP-immunoreactive axon usually provided 1-3 close appositions on individual ChAT- or TH-positive neurons. Serotonin-immunoreactive neurons were most heavily innervated, with the majority of raphé pallidus, raphé obscurus and parapyramidal neurons receiving several close appositions from large varicosities of YFP-immunoreactive axons. These results indicate that GLP-1 neurons innervate various populations of brainstem autonomic neurons. These include vagal efferent neurons and catecholamine neurons in areas linked with cardiovascular control. Our data also indicate a synaptic connection between GLP-1 neurons and 5-HT neurons, some of which might contribute to the regulation of appetite.
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影响因子：
2.9
作者：
Kopf, Brigitte S.;Langhans, Wolfgang;Asarian, Lori
通讯作者：
Asarian, Lori
影响因子：
3.3
作者：
Llewellyn-Smith, I. J.;Reimann, F.;Gribble, F. M.;Trapp, S.
通讯作者：
Trapp, S.
DOI：
10.1523/jneurosci.1809-08.2008
发表时间：
2008-10-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Cano G;Mochizuki T;Saper CB
通讯作者：
Saper CB
影响因子：
3.3
作者：
Larsen, PJ;TangChristensen, M;Orskov, C
通讯作者：
Orskov, C
影响因子：
4.8
作者：
Hayes, Matthew R.;Skibicka, Karolina P.;Grill, Harvey J.
通讯作者：
Grill, Harvey J.