A microfluidic technique for monitoring bloodstream analytes indicative of C-peptide resistance in type 2 diabetes.
A microfluidic technique for monitoring bloodstream analytes indicative of C-peptide resistance in type 2 diabetes.
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一种微流体技术，用于监测指示 2 型糖尿病 C 肽耐药性的血流分析物。
DOI：
10.1039/b816740k
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Spence,DanaM
中科院分区：
文献类型：
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作者：
Oblak,TeresaD'Amico;Meyer,JenniferA;Spence,DanaM
采用简单的聚二甲基硅氧烷（PDMS）微芯片建立红细胞（RBC）抗氧化状态与红细胞与金属活化c肽相互作用能力之间的关系，金属活化c肽是一种生物活性肽，据报道可减少糖尿病相关的一些并发症。众所周知，从2型糖尿病患者获得的红细胞中谷胱甘肽（GSH）的减少形式比从健康对照者获得的红细胞低，因此，这种相关性有可能涉及高危人群的2型糖尿病。平行通道微流控装置定量谷胱甘肽在年龄为基础的分数，随着控制和糖尿病红细胞描述。重要的是基于荧光的测量是同时测定抗氧化剂没有事先分离在任何一个六通道或十二通道微芯片。在这里，我们使用基于密度的Percoll溶液分离红细胞，并定量测定年轻、密度较低的红细胞中GSH的浓度（336.7±29.6 amol/RBC）比年龄较大、密度较高的红细胞（137.0±25.3 amol/RBC）增加2倍以上。通过免疫分析法测定了c肽与红细胞膜的相互作用能力，发现添加到年轻红细胞中的c肽的回收率比基础水平提高了40.6±12.7%以上，而添加到年老细胞中的c肽的回收率仅提高了9.18±4.60%。这些结果表明，红细胞中的谷胱甘肽浓度可能有助于筛选体内对c肽的耐药性。
A simple poly(dimethylsiloxane) (PDMS) microchip was employed to establish a relationship between red blood cell (RBC) antioxidant status and the ability of RBCs to interact with metal-activated C-peptide, a bio-active peptide reported to reduce some complications often associated with diabetes. It is known that the reduced form of glutathione (GSH) levels in the RBCs obtained from people with type 2 diabetes are lower in comparison to those RBCs obtained from healthy controls and accordingly, this correlation has the potential to implicate type 2 diabetes in high-risk individuals. A parallel channel microfluidic device for the quantification of GSH in age-based fractions, along with control and diabetic RBCs is described. Important to the fluorescence-based measurement is the simultaneous determination of the antioxidant without prior separation in either a six- or twelve-channel microchip. Here, we separated the RBCs using a density-based Percoll solution and quantitatively determined the concentration of GSH in younger, less dense RBCs to be increased more than 2-fold (336.7 ± 29.6 amol/RBC) than older, more dense RBCs (137.0 ± 25.3 amol/RBC). The ability of C-peptide to interact with the RBC membrane of the separated fractions was determined by immunoassay and it was found that the recovery of the C-peptide added to the younger RBCs increased by more than 40.6 ± 12.7% above basal levels while with the older cells C-peptide increased by only 9.18 ± 4.60%. These results suggest that GSH concentrations in the RBC may be useful in screening for resistance to C-peptidein vivo.