Gastrodia elata Blume water extracts improve insulin resistance by decreasing body fat in diet-induced obese rats: vanillin and 4-hydroxybenzaldehyde are the bioactive candidates
Gastrodia elata Blume water extracts improve insulin resistance by decreasing body fat in diet-induced obese rats: vanillin and 4-hydroxybenzaldehyde are the bioactive candidates
复制标题
DOI：
10.1007/s00394-010-0120-0
复制
发表时间：
2011-03-01
影响因子：
5
通讯作者：
Kang, Suna
中科院分区：
文献类型：
--
作者：
Park, Sunmin;Kim, Da Sol;Kang, Suna
胰岛素抵抗是肥胖、2型糖尿病和高脂血症等代谢性疾病的常见症状，我们研究了含有酚类化合物的天麻水提取物（GEB）是否对高脂饮食（HFD）喂养的雄性Sprague-Dawley大鼠的胰岛素抵抗具有有益作用，并确定了这种作用是如何产生的。另外，还鉴定了所涉及的生物活性候选物。喂食HFD的大鼠每天每公斤体重给予0.3g GEB（GEB-L）、1g GEB（GEB-H）或1g纤维素（对照）8周，而第四组大鼠喂食低脂饮食（LFD）。在体外实验中，GEB的4种主要成分被测试了对脂肪堆积的影响，对照组的大鼠表现出比喂食LFD的大鼠更高的附睾和腹膜后脂肪垫的重量增加，而GEB以剂量依赖的方式阻止这种增加。GEB-H显著降低能量摄入，部分是通过增强下丘脑STAT3磷酸化和减弱AMPK磷酸化。GEB-H也增加了能量消耗与脂肪氧化的增加。与对照组相比，GEB-H增加全身葡萄糖处置率并降低肝脏葡萄糖输出。在GEB的主要成分中，4-羟基苯甲醛和香草醛通过调节3T3-L1脂肪细胞中参与脂肪代谢的基因的表达来减少甘油三酯的积累。GEB-H主要是4-羟基苯甲醛和香草醛作用的结果，通过激活脂肪氧化和增强瘦素信号，减少脂肪细胞中脂肪的积累，从而降低胰岛素抵抗。
Insulin resistance is a common symptom of metabolic diseases such as obesity, type 2 diabetes and hyperlipidemia.We investigated whether Gastrodia elata Blume water extract(GEB), containing phenolic compounds, had a beneficial action on insulin resistance in male Sprague-Dawley rats fed a high fat diet(HFD) and determined how this effect was produced. In addition, the bioactive candidates involved were identified.Rats fed HFD were daily administered with 0.3 g GEB(GEB-L), 1 g GEB(GEB-H), or 1 g cellulose(control) per kg body weight for 8 weeks, while rats in the fourth group were fed a low fat diet(LFD). In vitro study, 4 major components of GEB were tested for their impact on fat accumulation.Rats in the control group exhibited a higher weight gain of epididymal and retroperitoneal fat pads than those fed LFD, while GEB prevented such an increment in a dose-dependent manner. GEB-H significantly decreased energy intake partly through potentiating STAT3 phosphorylation and attenuating AMPK phosphorylation in the hypothalamus. GEB-H also increased energy expenditure with the increase in fat oxidation. GEB-H increased whole body glucose disposal rates and decreased hepatic glucose output compared to the control. Among the major components of GEB, 4-hydroxybenzaldehyde and vanillin decreased triglyceride accumulation by modulating the expression of genes involved in fat metabolism in 3T3-L1 adipocytes. They increased insulin-stimulated glucose uptake to reduce insulin resistance.GEB-H, mainly as a result of the action of 4-hydroxybenzaldehyde and vanillin, reduces insulin resistance by decreasing fat accumulation in adipocytes by activating fat oxidation and potentiating leptin signaling in diet-induced obese rats.