Aptamer based fluorometric determination of ATP by exploiting the FRET between carbon dots and graphene oxide
Aptamer based fluorometric determination of ATP by exploiting the FRET between carbon dots and graphene oxide
复制标题
利用碳点和氧化石墨烯之间的 FRET 进行基于适体的荧光测定 ATP
DOI：
10.1007/s00604-018-2683-z
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发表时间：
2018-02-01
影响因子：
5.7
通讯作者：
Hu, Qin
中科院分区：
文献类型：
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作者：
Cheng, Xia;Cen, Yao;Hu, Qin
作者描述了一种基于荧光适体的三磷酸腺苷（ATP）测定方法。它基于碳点（CDs）和氧化石墨烯（GO）的使用。合成的cd -适体通过p-stacking和疏水相互作用吸附在氧化石墨烯表面，cd -适体的荧光通过cd和氧化石墨烯之间的荧光共振能量转移（FRET）被猝灭。如果存在ATP，它将与适配体结合，cd -适配体将从氧化石墨烯中解吸。这将抑制FRET和cd的荧光被恢复。在最佳条件下，在典型的激发/发射波长为358/ 455nm时，该方法具有80 pM的检测限和0.10 ~ 5.0 nm浓度范围内的线性范围。该方法成功地应用于酸奶样品中ATP的测定。这种方法也可以应用于其他有合适的适体的分析物的检测。
The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via p-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available.