Vascular endothelial growth factor (VEGF) mRNA splice variants are differentially expressed in human blastocysts
Vascular endothelial growth factor (VEGF) mRNA splice variants are differentially expressed in human blastocysts
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DOI：
10.1093/molehr/7.1.57
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发表时间：
2001-01-01
影响因子：
4
通讯作者：
Polan, ML
中科院分区：
文献类型：
--
作者：
Krüssell, JS;Behr, B;Polan, ML
本研究的目的是检测和表征人胚泡中VEGF（121）、VEGF（145）、VEGF（165）、VEGF（189）和VEGF（206）亚型的mRNA表达。我们最近证实了VEGF mRNA在人类着床前胚胎发育过程中的表达，为了更好地理解着床期间血管生成的过程，需要进一步了解选择性剪接mRNA导致自由分泌蛋白或与细胞表面硫酸肝素蛋白聚糖结合的蛋白。通过反转录/半巢式聚合酶链反应检测了从斯坦福大学体外受精项目获得的不适合移植的人囊胚的VEGF mRNA剪接变异体的表达。19个囊胚中有17个（89%）表达VEGF mRNA。在17个囊胚中，88%的囊胚检测到VEGF(121) mRNA， 100%的囊胚检测到VEGF(145) mRNA， 71%的囊胚检测到VEGF(165) mRNA， 24%的囊胚检测到VEGF(189) mRNA。VEGF（121）和VEGF(145) mRNA仅在29%囊胚中同时表达，VEGF（165）和VEGF(145) mRNA仅在12%囊胚中同时表达，59%囊胚中VEGF（121）、VEGF（145）和VEGF(165) mRNA均未检测到VEGF(206) mRNA。总之，我们证明囊胚表达编码游离VEGF蛋白的mrna，使植入胚胎能够在植入部位立即诱导血管生成。
The aim of our study was to detect and characterize mRNA expression of VEGF isoforms VEGF(121), VEGF(145), VEGF(165), VEGF(189), and VEGF(206) in human blastocysts. We recently demonstrated VEGF mRNA expression during human preimplantation embryo development, and further information regarding the alternatively spliced mRNAs resulting in freely secreted proteins or proteins bound to cell surface heparan-sulphate proteoglycans is needed to better understand the process of angiogenesis during implantation. Human blastocysts unsuitable for transfer obtained from the IVF programme at Stanford University were examined by reverse transcription/hemi-nested polymerase chain reaction for their expression of VEGF mRNA splice variants. VEGF mRNA was expressed in 17 out of 19 (89%) blastocysts. Of the 17 blastocysts, VEGF(121) mRNA was detected in 88%, VEGF(145) mRNA in 100%, VEGF(165) mRNA in 71%, and VEGF(189) mRNA in 24% of blastocysts. There was co-expression of mRNA for VEGF(121) and VEGF(145) only in 29% blastocysts, of mRNA for VEGF(165) and VEGF(145) only in 12%, and of mRNA for VEGF(121), VEGF(145) and VEGF(165) in 59% blastocysts, VEGF(206) mRNA could not be detected. In conclusion, we demonstrated that blastocysts express the mRNAs encoding for the free VEGF proteins, enabling the implanting embryo to immediately induce angiogenesis at the implantation site.