Polar cell fate stimulates Wolbachia intracellular growth.
Polar cell fate stimulates Wolbachia intracellular growth.
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DOI：
10.1242/dev.158097
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发表时间：
2018-03-23
期刊：
影响因子：
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通讯作者：
Frydman, Horacio M
中科院分区：
文献类型：
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作者：
Kamath, Ajit D;Deehan, Mark A;Frydman, Horacio M
细菌是脊椎动物和无脊椎动物发育和进化的重要伙伴。很大一部分昆虫携带沃尔巴克氏体，这是一种操纵宿主繁殖以促进其传播的细菌内共生体。因为它们是母系遗传的，沃尔巴克氏体在选择压力下到达雌性生殖细胞并感染后代。然而，沃尔巴克氏体感染并不限于生殖系。体细胞类型，包括干细胞龛，与周围组织相比具有更高的沃尔巴克氏体负载。在这里，我们展示了一个新的沃尔巴克氏体向性极细胞（PC），专门的体细胞在果蝇卵巢。在卵子发生过程中，PC发育的所有阶段都很容易可视化，便于研究沃尔巴克氏体细胞内生长的动力学。沃尔巴克氏体的积累是由PC形态发生的特定事件触发的，包括从祖细胞分化和卵子发生的第8和第9阶段之间的分化。此外，异位PC命运的诱导足以促进沃尔巴克氏体积累。我们发现沃尔巴克氏体PC嗜性在大多数果蝇物种中是进化保守的，但在库蚊中则不然。这些发现突出了内共生体的向性与宿主发育和细胞分化的协调。
Bacteria are crucial partners in the development and evolution of vertebrates and invertebrates. A large fraction of insects harbor Wolbachia, bacterial endosymbionts that manipulate host reproduction to favor their spreading. Because they are maternally inherited, Wolbachia are under selective pressure to reach the female germline and infect the offspring. However, Wolbachia infection is not limited to the germline. Somatic cell types, including stem cell niches, have higher Wolbachia loads compared with the surrounding tissue. Here, we show a novel Wolbachia tropism to polar cells (PCs), specialized somatic cells in the Drosophila ovary. During oogenesis, all stages of PC development are easily visualized, facilitating the investigation of the kinetics of Wolbachia intracellular growth. Wolbachia accumulation is triggered by particular events of PC morphogenesis, including differentiation from progenitors and between stages 8 and 9 of oogenesis. Moreover, induction of ectopic PC fate is sufficient to promote Wolbachia accumulation. We found that Wolbachia PC tropism is evolutionarily conserved across most Drosophila species, but not in Culex mosquitos. These findings highlight the coordination of endosymbiont tropism with host development and cell differentiation.