YAP regulates periodontal ligament cell differentiation into myofibroblast interacted with RhoA/ROCK pathway
YAP regulates periodontal ligament cell differentiation into myofibroblast interacted with RhoA/ROCK pathway
复制标题
YAP 与 RhoA/ROCK 通路相互作用调节牙周膜细胞分化为肌成纤维细胞
DOI：
10.1002/jcp.27312
复制
发表时间：
2019-04-01
影响因子：
5.6
通讯作者：
Bai, Ding
中科院分区：
文献类型：
--
作者：
He, Yao;Xu, Hui;Bai, Ding
在正畸牙齿移动过程中，牙周膜细胞（PDLCs）接受机械刺激并转化为肌成纤维细胞（Mfbs）。事实上，以前的研究已经证明，机械刺激可以促进PDLCs中Mfb标志物α-平滑肌肌动蛋白（α-SMA）的表达。转化生长因子β1（Transforming growth factor β1，TGF-β1）作为眼相关蛋白（yes-associated protein，雅普）的靶基因参与了这一过程。在此，我们试图评估雅普在Mfbs从PDLC分化中的作用。雅普和α-SMA在体内OTM模型中以及在体外张力刺激下的时程表达均得到证实。使用Y27632抑制RhoA/Rho相关激酶（ROCK）途径显著降低了张力诱导的Mfb分化和雅普表达。此外，在PDLCs中过表达雅普并用慢病毒转染挽救了由Y27632诱导的Mfb分化的抑制作用。这些数据共同表明雅普在调节张力诱导的Mfb从与RhoA/ROCK通路相互作用的PDLC分化中的关键作用。
During orthodontic tooth movement (OTM), periodontal ligament cells (PDLCs) receive the mechanical stimuli and transform it into myofibroblasts (Mfbs). Indeed, previous studies have demonstrated that mechanical stimuli can promote the expression of Mfb marker α‐smooth muscle actin (α‐SMA) in PDLCs. Transforming growth factor β1 (TGF‐β1), as the target gene of yes‐associated protein (YAP), has been proven to be involved in this process. Here, we sought to assess the role of YAP in Mfbs differentiation from PDLCs. The time‐course expression of YAP and α‐SMA was manifested in OTM model in vivo as well as under tensional stimuli in vitro. Inhibition of RhoA/Rho‐associated kinase (ROCK) pathway using Y27632 significantly reduced tension‐induced Mfb differentiation and YAP expression. Moreover, overexpression of YAP with lentiviral transfection in PDLCs rescued the repression effect of Mfb differentiation induced by Y27632. These data together suggest a crucial role of YAP in regulating tension‐induced Mfb differentiation from PDLC interacted with RhoA/ROCK pathway.