Determination of five arsenic species in aqueous samples by HPLC coupled with a hexapole collision cell ICP-MS
Determination of five arsenic species in aqueous samples by HPLC coupled with a hexapole collision cell ICP-MS
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DOI：
10.1039/b202172b
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发表时间：
2002-09
影响因子：
3.4
通讯作者：
Q. Xie;R. Kerrich;Elaine C. Irving;K. Liber;F. Abou-Shakra
中科院分区：
文献类型：
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作者：
Q. Xie;R. Kerrich;Elaine C. Irving;K. Liber;F. Abou-Shakra
建立了一种同时测定水样中砷钒、甲基砷（MMA）、二甲基砷（DMA）、砷Ⅲ和砷甜菜碱（AsB）的新方法。该方法使用了与六极碰撞池电感耦合等离子体质谱仪（ICP-MS）耦合的多模式离子交换柱。使用10 mM NH 4 NO3和0.05% HNO 3的混合物作为移动的相，通过以等度模式运行的高效液相色谱（HPLC）泵以0.4 mL min-1的流速泵送。在这些条件下，5种As在14 min内分离。柱出口通过低流量Meinhard同心雾化器直接连接至ICP-MS。河水认证的参考物质（SLRS-4），加标五种As物质，每种20 µg L−1（As阳离子），用于验证真实的样品基质中的色谱分离和定量。基于SLRS-4的重复分析，精密度范围为AsV的2%至MMA的10%。检测限（3s）范围为0.02 µg L−1（AsB）至0.4 µg L−1（MMA）。新方法被应用于水样中收集的毒性试验实验。结果表明，在毒性试验过程中，无机砷形态（AsV和AsIII）发生了转化，但未检测到无机砷形态的甲基化。
A new method has been developed for the simultaneous determination of AsV, monomethylarsenic (MMA), dimethylarsenic (DMA), AsIII, and arsenobetaine (AsB) in aqueous samples. The method utilizes a multi-mode ion exchange column coupled with a hexapole collision cell inductively coupled plasma mass spectrometer (ICP-MS). A mixture of 10 mM NH4NO3 and 0.05% HNO3 was used as the mobile phase, pumped by a high-performance liquid chromatography (HPLC) pump running in an isocratic mode at a flow rate of 0.4 mL min−1. Under these conditions, the five As species were separated within 14 min. The column outlet was connected directly to the ICP-MS via a low flow Meinhard concentric nebulizer. A river water certified reference material (SLRS-4), spiked with five As species at 20 µg L−1 (As cation) each, was used to validate the chromatographic separation and quantification in a real sample matrix. Based on replicate analyses of SLRS-4, the precision varied from 2% for AsV to 10% for MMA. The detection limits (3s) ranged from 0.02 µg L−1 for AsB to 0.4 µg L−1 for MMA. The new method was applied to water samples collected during As toxicity test experiments. The results indicated that transformation of inorganic As species (AsV and AsIII) occurred during the toxicity test experiments, but no methylation of inorganic As species was detected.