Bone marrow grafts restore cerebral blood flow and blood brain barrier in stroke rats
Bone marrow grafts restore cerebral blood flow and blood brain barrier in stroke rats
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DOI：
10.1016/j.brainres.2004.02.072
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发表时间：
2004-06-04
期刊：
影响因子：
2.9
通讯作者：
Hess, DC
中科院分区：
文献类型：
--
作者：
Borlongan, CV;Lind, JG;Hess, DC
我们监测了大脑中动脉闭塞（MCAo）和纹状体内移植小鼠骨髓基质细胞（BMSCs）或生理盐水输注后成年Sprague-Dawley大鼠脑血流量（CBF）和血脑屏障（BBB）通透性的变化。激光多普勒和伊文思蓝分析显示，与接受盐水输注的中风动物（MCAo后11-14天）相比，在移植的中风动物中，BMSC移植物在更早的时期（MCAo后4-5天）密切依赖地将CBF和BBB恢复到接近正常水平。异种骨髓间充质干细胞在没有免疫抑制的情况下存活，并且在移植的中风动物中检测到转化生长因子-β神经营养因子超家族水平升高。这些数据表明，移植BMSCs后CBF和BBB的早期恢复可以介导中风动物的功能结果。(C)2004 Elsevier B. V.保留所有权利。
We monitored alterations in cerebral blood flow (CBF) and blood-brain barrier (BBB) permeability following middle cerebral artery occlusion (MCAo) and intrastriatal transplantation of mouse bone marrow stromal cells (BMSCs) or saline infusion in adult Sprague-Dawley rats. Laser Doppler and Evans Blue assay revealed that BMSC grafts close-dependently restored CBF and BBB to near normal levels at a much earlier period (Days 4-5 post-MCAo) in transplanted stroke animals compared to stroke animals that received saline infusion (Days 11-14 post-MCAo). Xenogratted BMSCs survived in the absence of immunosuppression, and elevated levels of transforming growth factor-beta superfamily of neurotrophic factors were detected in transplanted stroke animals. These data suggest that early restoration of CBF and BBB following transplantation of BMSCs could mediate the reported functional outcomes in stroke animals. (C) 2004 Elsevier B.V. All rights reserved.