Analysis of Catharanthus roseus alkaloids by HPLC
Analysis of Catharanthus roseus alkaloids by HPLC
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DOI：
10.1007/s11101-006-9036-y
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发表时间：
2007-07-01
影响因子：
7.7
通讯作者：
Jolicoeur, Mario
中科院分区：
文献类型：
--
作者：
Hisiger, Steve;Jolicoeur, Mario
玫瑰花是一种药用植物，从玫瑰花中提取次生代谢物，用于化疗治疗多种癌症。众所周知的高价值代谢产物长春新碱和长春花碱只是在玫瑰中发现的130种生物碱中的2种。然而，在这么多的化学实体中，只有少数（大约11种）经常被分析，甚至更少（大约8种）可以在商业上获得。30多年来，人们发展了不同的分析技术来分离和鉴定玫瑰金针菇代谢物，从而揭示玫瑰金针菇代谢物的治疗潜力。在众多的方法中，高效液相色谱（HPLC）技术仍被广泛用于分离和分析次生代谢物，如玫瑰花中的次生代谢物。本文综述了玫瑰中生物碱的高效液相色谱分析的最新进展。详细回顾了对二级代谢物分离和鉴定步骤效率至关重要的各种考虑因素，如生物质操作，提取阶段和方案，高效液相色谱分离和分析方案。用最常见的检测器获得的光谱的例子也显示，并建议如何继续发展有效的分离和鉴定方法在分析和半制备尺度。
Catharanthus roseus is a medicinal plant from which secondary metabolites used in chemotherapy to treat diverse cancers are extracted. The well known high value metabolites vincristine and vinblastine are just 2 of 130 alkaloids that can be found in C. roseus. However, only few (similar to 11) of this high number of chemical entities are frequently analyzed and even fewer (similar to 8) are available commercially. For more than 30 years, different analytical techniques have been developed to isolate and identify C. roseus metabolites, and then allowing revealing the therapeutic potential of C. roseus metabolites. Among few approaches, high performance liquid chromatography (HPLC) technique is still widely used for the separation and analysis of secondary metabolites such as those from C. roseus. This article thus reviews the most recent developments in HPLC analysis of alkaloids from C. roseus. Diverse considerations that are crucial to the efficiency of secondary metabolites separation and identification steps, such as biomass manipulation, extraction phase and protocols, HPLC separation and analysis protocols are reviewed in details. Examples of spectra obtained using the most common detectors are also shown and suggestions are made on how to proceed in developing efficient separation and identification methods at the analytical and semi-preparative scales.