Gongronella sp induces overproduction of laccase in Panus rudis
Gongronella sp induces overproduction of laccase in Panus rudis
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DOI：
10.1002/jobm.200900155
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发表时间：
2010-02-01
影响因子：
3.1
通讯作者：
Xiao, Yazhong
中科院分区：
文献类型：
--
作者：
Wei, Fen;Hong, Yuzhi;Xiao, Yazhong
漆酶通常是通过化学诱导产生的，也是由寄主与典型的生防木霉属相互作用合成的。在本研究中，我们发现了一株新分离的非漆酶产生菌Gongronella sp.W5能诱导红腹黑松茸产生过量的漆酶。该酶的活力为148,200UI-1，是以铜/邻甲基苯胺为诱导剂的化学诱导法的25倍。分离纯化了鲁氏假单胞菌与G.W5相互作用产生的一种新的漆酶同工酶，并对其进行了鉴定。进一步的实验表明，G.W5分泌的一些耐pH代谢产物可以作为信号诱导鲁氏假单胞菌漆酶。漆酶在Trametes sp.中也有较高的表达。AH28-2与木霉相互作用。ZH1。然而，从鲁迪氏假丝酵母与木霉的交叉作用中没有观察到漆酶活性。ZH1或Trametes sp.AH28-2-GW5.
Laccase is usually produced via chemical induction and is also synthesized by hosts in interaction with the typical bio-control genus Trichoderma. In this study, we found that a newly isolated non-laccase-producing fungus, Gongronella sp. W5, could induce overproduction of laccase in Panus rudis. The enzyme activity, 148,200 U I-1, was 25 times higher than the activity obtained from a chemical induction using copper/0-toluidine as inducers. A new laccase isozyme from the interaction of P. rudis and G. W5 was purified and characterized. A further test showed that some pH resistant metabolites secreted by G. W5 acted as signals to induce P. rudis laccase. Laccase is also highly expressed by Trametes sp. AH28-2 in interaction with Trichoderma sp. ZH1. However, no laccase activity was observed from the cross-over interactions of P. rudis-Trichoderma sp. ZH1 or Trametes sp. AH28-2-G. W5.