Hepatic expression of toll-like receptor 4 in primary biliary cirrhosis
Hepatic expression of toll-like receptor 4 in primary biliary cirrhosis
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DOI：
10.1016/j.jaut.2005.05.003
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发表时间：
2005-08-01
影响因子：
12.8
通讯作者：
Ishibashi, H
中科院分区：
文献类型：
--
作者：
Wang, AP;Migita, K;Ishibashi, H
Toll样受体4(TLR4)是细菌脂多糖的受体，可能参与肝胆管疾病的发病机制。为了探讨该受体在原发性胆汁性肝硬变(PBC)中的可能作用，我们研究了PBC患者肝组织中TLR4的表达。我们研究了62例PBC患者和41例慢性丙型肝炎(CHC)患者的肝活检切片。免疫组织化学方法检测TLR4在石蜡包埋切片中的表达。PBC肝组织的胆管上皮细胞表达TLR4，而CHC肝组织的胆管上皮细胞几乎不表达TLR4。TLR4在PBC肝组织门脉周围肝细胞中也有表达，晚期PBC肝组织中TLR4表达延伸至小叶间肝细胞。尽管CHC肝组织门脉周围肝细胞表达TLR4，但其表达水平与肝纤维化分期无关。结果表明，TLR4在PBC肝脏的BECs和门静脉周围肝细胞中有表达，提示细菌和TLR4可能参与了PBC的炎症过程。(C)2005爱思唯尔有限公司。保留所有权利。
Toll-like receptor 4 (TLR4) is a receptor for bacterial lipopolysaccharide, which is suggested to be involved in the pathogenesis of disease of hepatobiliary tracts. To explore a possible role for this receptor in primary biliary cirrhosis (PBC), we investigated the expression of TLR4 in liver tissues from PBC patients. We studied liver biopsy sections from 62 PBC patients and 41 patients with chronic hepatitis C (CHC). Expression of TLR4 in paraffin-embedded sections was analyzed by immunohistochemistry. The bile duct epithelial cells (BECs) of PBC liver tissues markedly expressed TLR4, whereas BECs of, CHC liver tissues barely expressed TLR4. The TLR4 expression was also observed in periportal hepatocytes of PBC liver tissues and its expression was extended to interlobular hepatocytes in advanced stage PBC. Although periportal hepatocytes of CHC liver tissues expressed TLR4, its expression levels were not correlated with the fibrosis stage. Our data demonstrated that TLR4 was expressed in BECs and periportal hepatocytes in PBC livers, suggesting the possible involvement of bacterial pathogens and TLR4 in the inflammatory processes of PBC. (c) 2005 Elsevier Ltd. All rights reserved.