A simple and rapid decalcification procedure of skeletal tissues for pathology using an ultrasonic cleaner with D-mannitol and formic acid
A simple and rapid decalcification procedure of skeletal tissues for pathology using an ultrasonic cleaner with D-mannitol and formic acid
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DOI：
10.1016/j.acthis.2014.01.006
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Imura, Johji
中科院分区：
文献类型：
--
作者：
Hatta, Hideki;Tsuneyama, Koichi;Imura, Johji
为了制备硬组织（如骨和牙齿）的组织病理学制剂，需要进行脱钙程序。脱钙通常通过将硬组织浸入具有各种性质的不同脱钙液中来进行。这些脱钙流体通常包括无机酸和有机酸、含有螯合剂的中性流体或溶液的混合物。不幸的是，没有一种通用的脱钙液能满足病理学家的所有要求，如快速脱钙、易于处理和最小的组织损伤。涉及使用微波（MW）或超声波设备（US）的技术已被证明可用于缩短脱钙程序的时间。在本研究中，我们调查了一个独特的脱钙过程，使用一个常见的商业超声波清洗器和脱钙液（甲酸）含有自由基清除剂（D-甘露醇）。完成该程序所需的时间大约是完成标准脱钙程序所需时间的一半。此外，脱钙后组织形态和抗原性保存得相当好。该手术快速，易于执行，并以最小的组织损伤实现硬组织脱钙。(C)2014 Elsevier GmbH. All rights reserved.
Decalcification procedures are required in order to prepare histopathological preparations of hard tissues such as bone and teeth. Decalcification is usually performed by immersing the hard tissue in different decalcification fluids with various properties. These decalcification fluids typically include inorganic and organic acids, a neutral fluid containing a chelating agent, or a mixture of solutions. Unfortunately, there is no universal decalcification fluid that satisfies all the requirements of pathologists such as rapid decalcification, easy handling, and minimal tissue damage. Techniques involving use of microwaves (MW) or ultrasonic apparatus (US) have been shown to be useful for shortening the time for decalcification procedures. In the present study, we investigated a unique decalcification procedure that uses a common commercial ultrasonic cleaner and a decalcification fluid (formic acid) containing a free-radical scavenger (D-mannitol). The time required to complete the procedure is approximately half of that required to complete a standard decalcification procedure. In addition, tissue morphology and antigenicity is fairly well preserved after decalcification. The procedure is quick, easy to perform, and achieves decalcification of hard tissue with minimal tissue damage. (C) 2014 Elsevier GmbH. All rights reserved.