Interplay between spinodal decomposition and glass formation in proteins exhibiting short-range attractions
Interplay between spinodal decomposition and glass formation in proteins exhibiting short-range attractions
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DOI：
10.1103/physrevlett.99.118301
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发表时间：
2007-09-14
影响因子：
8.6
通讯作者：
Schurtenberger, Peter
中科院分区：
文献类型：
--
作者：
Cardinaux, Frederic;Gibaud, Thomas;Schurtenberger, Peter
我们利用球形蛋白溶菌酶的水溶液作为具有短程吸引力的胶体的模型系统，研究了spinodal分解和动态阻滞之间的竞争。我们表明，温度低于T-a的淬火导致凝胶形成，这是由于在spinodal分解过程中蛋白质致密相的局部停止。这些凝胶的流变特性使我们能够使用离心实验来确定两相的局部密度，并精确定位凝胶边界和吸引玻璃线靠近相图的不稳定区域。
We investigate the competition between spinodal decomposition and dynamical arrest using aqueous solutions of the globular protein lysozyme as a model system for colloids with short-range attractions. We show that quenches below a temperature T-a lead to gel formation as a result of a local arrest of the protein-dense phase during spinodal decomposition. The rheological properties of these gels allow us to use centrifugation experiments to determine the local densities of both phases and to precisely locate the gel boundary and the attractive glass line close to and within the unstable region of the phase diagram.