Immunofluorescent studies for alpha-actinin in cultured cardiomyopathic hamster heart cells.
Immunofluorescent studies for alpha-actinin in cultured cardiomyopathic hamster heart cells.
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培养的心肌病仓鼠心脏细胞中α-辅肌动蛋白的免疫荧光研究。
DOI：
10.1002/ar.1092280108
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Lemanski,LF
中科院分区：
文献类型：
--
作者：
Li,JA;Lemanski,LF
正常和遗传性心肌病 (CM) 新生仓鼠（UM X7.1 品系）的心肌细胞原代培养物在培养 3、5、7 和 9 天后通过间接免疫荧光显微镜进行分析。将培养物固定在冷丙酮中，并通过间接方法使用 FITC 标记的抗 α-辅肌动蛋白来标记肌原纤维 Z 带进行免疫染色。大多数正常和 CM 肌细胞在培养 3 天后呈现圆形。正常心肌细胞在培养 5 天后开始表现出细胞质投射，并且它们的肌原纤维通常表现出彼此平行的排列。来自 CM 心脏的心肌细胞显示出明显的肌原纤维紊乱。此外，突起的形成比正常情况要晚。随着细胞大小的增加，正常仓鼠肌细胞在发育过程中形成越来越多的突起。相比之下，大多数心肌病肌细胞即使在培养9天后也几乎没有显示出投射。因此，心肌病肌细胞中每个细胞的投射数量比正常肌细胞少得多，尤其是在培养 7 天和 9 天后。这些结果表明，心肌病细胞在培养物中具有异常形状，特别是无法像正常细胞那样形成突起。这种异常行为是否与心肌病心脏细胞的膜或细胞骨架系统异常或其他一些因素有关，需要进一步研究。
Primary cultures of cardiac myocytes from normal and genetically cardiomyopathic (CM) newborn hamsters (strain UM X7.1) were analyzed by indirect immunofluorescent microscopy after 3, 5, 7, and 9 days in culture. The cultures were fixed in cold acetone and immunostained by an indirect method using FITC‐labelled anti‐α‐actinin to label the myofibrillar Z bands. Most normal and CM myocytes appeared round in shape after 3 days in culture. Normal cardiac myocytes began to exhibit cytoplasmic projections after 5 days in culture and their myofibrils usually showed parallel arrangements with respect to each other. The cardiac cells from CM hearts showed an obvious myofibril disarray. Moreover, projections formed later than normal. As the size of the cells increased, more and more projections formed in normal hamster myocytes during development. By contrast, most of the cardiomyopathic myocytes showed few projections even as late as 9 days in culture. Hence, the number of projections per cell was much less in cardiomyopathic myocytes than in normal, especially after 7 and 9 days in culture. These results suggest that cardiomyopathic cells have abnormal shapes in culture and, in particular, fail to form projections as in normal cells. Whether this unusual behavior is related to an abnormality of the membranes or cytoskeletal system in cardiomyopathic heart cells or to some other factor requires further study.