The Rb family contains a conserved cyclin-dependent-kinase-regulated transcriptional repressor motif.
The Rb family contains a conserved cyclin-dependent-kinase-regulated transcriptional repressor motif.
复制标题
Rb 家族包含保守的细胞周期蛋白依赖性激酶调节转录抑制基序。
DOI：
10.1128/mcb.16.12.7173
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发表时间：
1996
影响因子：
5.3
通讯作者：
Dean,DC
中科院分区：
文献类型：
--
作者：
Chow,KN;Starostik,P;Dean,DC
Progression through the cell cycle is dependent on the sequential expression of cyclins, which combine with cyclin-dependent kinases (cdks) to form active kinases. The transition from G1to S phase is dependent on D cyclins in complex with cdk4 or cdk6 and cyclin E complexed with cdk2. One target of G1cyclins is the retinoblastoma susceptibility protein (Rb). Rb is a transcriptional repressor that is selectively targeted to genes through interaction with the E2F family of cell cycle transcription factors. Rb is a member of a family of proteins that include p107 and p130. The three proteins share a region known as the pocket that is important for binding E2F and is also the binding site for oncoproteins from DNA tumor viruses that inactivate Rb. We have found that two conserved domains within the Rb pocket (A and B) interact to form a transcriptional repressor motif (K. N. B. Chow and D. C. Dean, Mol. Cell. Biol. 16:4862–4868, 1996). Here we demonstrate that p107 also has an A-B repressor motif, and using domain swapping and coimmunoprecipitation assays, we compare A and B from Rb and p107. Finally and most importantly, we demonstrate that the A-B interaction which forms the repressor motif is blocked by G1cdk phosphorylation, thereby blocking repressor activity. This A-B repressor motif is then the first example of a cdk-regulated transcriptional repressor.
