Comparison of the recovery of Mycobacterium bovis isolates using the BACTEC MGIT 960 system, BACTEC 460 system, and Middlebrook 7H10 and 7H11 solid media
Comparison of the recovery of Mycobacterium bovis isolates using the BACTEC MGIT 960 system, BACTEC 460 system, and Middlebrook 7H10 and 7H11 solid media
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DOI：
10.1177/104063870601800302
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发表时间：
2006-05-01
影响因子：
1.5
通讯作者：
Hoffman, LJ
中科院分区：
文献类型：
--
作者：
Hines, N;Payeur, JB;Hoffman, LJ
对BACTEC微细菌生长指示管（MGIT）960系统进行了评价，以确定其与BACTEC 460辐射测定系统和固体培养基在从组织样本中回收牛分枝杆菌方面的比较情况。2003年11月至2004年9月期间，从美国和墨西哥的屠宰场共收集了506份牛淋巴结样本。将处理后的样品接种到MGIT 960试管、BACTEC 460小瓶以及Middlebrook 7 H10和Middlebrook 7 H11固体培养基中。制备Ziehl-Neelsen玻片，以检查污染物并确认是否存在抗酸阳性杆菌。含有抗酸杆菌的样品通过核酸测定被确认为结核分枝杆菌复合体的成员。用烟酸和硝酸盐生化试验鉴别M. bovis从M.结核病分离株。进行了统计分析，以比较回收率、平均检出时间、污染率以及每个类别的成对比较。结果表明，MGIT 960系统对M. Bovis（122/129）比BACTEC 460（102/129）和固体培养基系统（96/129）更高。MGIT 960系统的平均检测时间为15.8天，BACTEC 460系统为28.2天，固体培养基为43.4天。MGIT 960系统的污染率为6.9%，BACTEC 460系统为3.4%，固体培养基为21.7%。这些结果表明，MGIT 960系统可以作为BACTEC 460系统的替代品用于回收M。牛的组织样本。
The BACTEC Microbacteria Growth Indicator Tube (MGIT) 960 system was evaluated to determine how it compares with the BACTEC 460 radiometric system and solid media for recovery of Mycobacterium bovis from tissue samples. A total of 506 bovine lymph node samples were collected from abattoirs in the United States and Mexico between November 2003 and September 2004. Processed samples were inoculated into an MGIT 960 tube, BACTEC 460 vial, and Middlebrook 7H10 and Middlebrook 7H11 solid media. Ziehl-Neelsen slides were prepared to check for contaminants and confirm the presence of acid-fast positive bacilli. Samples containing acid-fast bacilli were confirmed as members of the Mycobacterium tuberculosis complex by a nucleic acid assay. Niacin and nitrate biochemical tests were used to distinguish M. bovis from M. tuberculosis isolates. Statistical analyses were performed to compare recovery rate, mean time to detection, contamination rates, as well as pair-wise comparisons in each category. The results showed that the MGIT 960 system had a higher recovery rate of M. bovis (122/129) than did the BACTEC 460 (102/129) and solid media system (96/129). The average time to detection was 15.8 days for the MGIT 960 system, 28.2 days for the BACTEC 460 system, and 43.4 days for solid media. Contamination rates were 6.9% for the MGIT 960 system, 3.4% for the BACTEC 460 system, and 21.7% for solid media. These results indicate the MGIT 960 system can be used as an alternative to the BACTEC 460 system for recovering M. bovis from tissue samples.