Adipose-derived stem cells induce vascular tube formation of outgrowth endothelial cells in a fibrin matrix
Adipose-derived stem cells induce vascular tube formation of outgrowth endothelial cells in a fibrin matrix
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DOI：
10.1002/term.1620
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发表时间：
2015-02-01
影响因子：
3.3
通讯作者：
Redl, Heinz
中科院分区：
文献类型：
--
作者：
Holnthoner, Wolfgang;Hohenegger, Karin;Redl, Heinz
工程组织的血管化是当前组织工程的挑战之一。几种策略旨在产生一种可植入损伤或创伤部位的预血管支架。来源于外周血的内皮细胞（外生内皮细胞，OECs）在血管组织工程中显示出很有前途的特性，包括它们的自体性质、增殖能力和形成成熟血管的能力。在这项研究中，我们研究了脂肪源性干细胞在纤维蛋白基质中与脂肪源性干细胞（ASCs）共培养形成血管结构的能力。使用包被oec的微载体珠，我们在纤维蛋白支架中显示了内皮细胞的向内生长。此外，CD31免疫染色证实，与ASCs共培养可诱导血管形成。纤维蛋白的降解至少部分是由基质金属蛋白酶-14的表达介导的。此外，我们还发现，即使在没有微载体珠粒的情况下，OEC/ asc诱导的血管样结构也会形成，其中asc数量的增加导致了更密集的管状网络。我们的数据为在自体系统中纤维蛋白基质内共培养诱导的外生内皮细胞血管形成提供了新的见解。版权所有：John Wiley & Sons， Ltd。
Vascularization of engineered tissues is one of the current challenges in tissue engineering. Several strategies aim to generate a prevascularized scaffold which can be implanted at sites of injury or trauma. Endothelial cells derived from peripheral blood (outgrowth endothelial cells, OECs) display promising features for vascular tissue engineering, including their autologous nature, capacity for proliferation and ability to form mature vessels. In this study we investigated the ability of OECs to form vascular structures in co-culture with adipose-derived stem cells (ASCs) in a fibrin matrix. Using microcarrier beads coated with OECs, we showed ingrowth of endothelial cells in the fibrin scaffold. Furthermore, co-cultures with ASCs induced vessel formation, as evidenced by immunostaining for CD31. The degradation of fibrin is at least in part mediated by expression of matrix metalloproteinase-14. Moreover, we showed OEC/ASC-induced vessel-like structure formation even in the absence of microcarrier beads, where increasing amounts of ASCs resulted in a denser tubular network. Our data add new insights into co-culture-induced vessel formation of outgrowth endothelial cells within a fibrin matrix in an autologous system. Copyright (c) 2012 John Wiley & Sons, Ltd.