QUANTITATIVE-ANALYSIS OF DISTURBED CELL MATURATION IN DYSPLASTIC LESIONS OF THE RESPIRATORY-TRACT EPITHELIUM
QUANTITATIVE-ANALYSIS OF DISTURBED CELL MATURATION IN DYSPLASTIC LESIONS OF THE RESPIRATORY-TRACT EPITHELIUM
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DOI：
10.1093/carcin/1.12.1007
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发表时间：
1980-01-01
期刊：
影响因子：
4.7
通讯作者：
OLSON, AC
中科院分区：
文献类型：
--
作者：
KLEINSZANTO, AJP;NETTESHEIM, P;OLSON, AC
在大鼠气管移植物中由[致癌物]7,12-二甲基苯并[a]蒽(DMBA)诱导的一组上皮损伤中，分析了[3H]胸苷掺入、核/细胞质比(N/C)和深色上皮细胞百分比的放射自显影模式[用光学显微镜观察]。不同年龄的相似病变表现出相同的标记指数（LI）。分析无或仅有轻度异型性的鳞状化生、中度异型性的鳞状化生、重度异型性的鳞状化生、原位癌微浸润癌、正常气管和食管上皮。尽管正常气管基底层表现出 LI < 1%，但检测到癌 (LI 约 32%) 和所有剩余上皮细胞 (LI 约 18%) 之间存在明显差异。基底上细胞的 LI 在无异型性的鳞状上皮 (LI 约 2%) 和包含所有非典型病变的组 (LI 约 9%) 之间表现出统计学上显着的差异。随着病变严重程度的增加，特别是在中层和表层，观察到 N/C（核-细胞质比）值的梯度增加。正常食管上皮中不存在暗细胞，规则鳞状上皮化生基底层中存在中等数量的暗细胞（18%），并且在非典型上皮病变中显着增加。在基底上层中，暗细胞从中度异型性鳞状化生中的3%增加到重度异型性化生中的28%和原位癌中的56%。细胞成熟和细胞增殖的紊乱是化学致癌剂引起的发育异常病变的主要特征。建议使用客观参数对癌前改变进行评估和分类。
Autoradiographic patterns [seen with light microscopy] of [3H]thymidine incorporation, nuclear/cytoplasmic ratios (N/C) and the percentage of dark epithelial cells were analyzed in a group of epithelial lesions induced by [the carcinogen] 7,12-dimethylbenz[a]anthracene (DMBA) in rat tracheal transplants. Similar lesions of different age exhibit the same labeling indices (LIs). Squamous metaplasia without or with only mild atypia, squamous metaplasia with moderate atypia, squamous metaplasia with severe atypia, carcinoma in situ microinvasive carcinoma, normal tracheal and esophageal epithelial were analyzed. Whereas the normal tracheal basal layer exhibited an LI < 1%, a clear difference between the carcinomas (LI .apprx. 32%) and all the remaining epithelial (LI .apprx. 18%) was detected. The LIs of the suprabasal cells exhibited a statistically significant difference between the squamous epithelia without atypia (LI .apprx. 2%) and the group comprising all the atypical lesions (LI .apprx. 9%). Gradients of increasing N/C (nucleus-cytoplasm ratios) values were observed as the lesions increased in severity, especially in the middle and surface layers. Dark cells were absent in the normal esophageal epithelium, were present in moderate numbers in the basal layer of regular squamous metaplasia (18%), and increased markedly in the atypical epithelial lesions. In the suprabasal layer dark cells increased from 3% in squamous metaplasia with moderate atypia to 28% in metaplasia with severe atypia and 56% in carcinoma in situ. Disturbances of cell maturation and cell proliferation are key features of dysplastic lesions induced by chemical carcinogens. The use of objective parameters for evaluation and classification of preneoplastic alterations is suggested.