Sialic acids in gastropods
Sialic acids in gastropods
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DOI：
10.1016/s0014-5793(01)03024-1
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发表时间：
2001-11-09
期刊：
影响因子：
3.5
通讯作者：
Staudacher, E
中科院分区：
文献类型：
--
作者：
Bürgmayr, S;Grabher-Meier, H;Staudacher, E
通过十二烷基硫酸钠电泳蛋白质，然后用唾液酸特异性凝集素染色的凝集素印迹法，测定了蛞蝓Lusitanicus（腹足纲）制剂中N-乙酰神经氨酸和N-羟乙酰神经氨酸残留物的发生。Maackia amurensis，通过这种结合对产气荚膜梭菌唾液酸酶的敏感性，通过用1，2-二氨基-4，5-亚甲二氧基苯特异性荧光标记唾液酸，通过测定对唾液酸-丙酮酸-裂解酶的敏感性，通过与标准品在高效阴离子交换色谱-脉冲安培检测器上共迁移，并通过鉴定三甲基甲硅烷基衍生物，在气相色谱法之后。这是首次在腹足类中发现唾液酸。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
The occurrence of N-acetylneuraminic acid and N- glycolylneuraminic acid residues in preparations of the slug Arion lusitanicus (Gastropoda) was determined by sodium dodecyl sulphate electrophoresis of the proteins followed by lectin blots stained with the sialic acid specific lectin from. Maackia amurensis, by the sensitivity of this binding to sialidase from Clostridium perfringens, by specific fluorescent labelling of sialic acids with 1,2-diamino-4,5-methylenedioxy benzene, by the determination of the sensitivity to sialate-pyruvate-lyase, by comigration with standards on high performance anion exchange chromatography with pulsed amperometric detection and by identification of the typical masses in the fragmentation patterns of the trimethylsilyl derivatives after gas chromatography. It is the first time sialic acids are identified in gastropods. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.