Unique ability of activated CD4+ T cells but not rested effectors to migrate to non-lymphoid sites in the absence of inflammation
Unique ability of activated CD4+ T cells but not rested effectors to migrate to non-lymphoid sites in the absence of inflammation
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DOI：
10.1074/jbc.m608266200
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发表时间：
2007-03-02
影响因子：
4.8
通讯作者：
Swain, Susan L.
中科院分区：
文献类型：
--
作者：
Agrewala, Javed N.;Brown, Deborah M.;Swain, Susan L.
最近的研究表明，免疫反应产生的效应 T 细胞会迁移到多个非淋巴部位，甚至是那些没有明显抗原表达或炎症的部位。为了研究不同的CD4(+) T淋巴细胞亚群进入并在非淋巴、非炎症区室中持续存在的能力，我们检测了体外产生的幼稚、效应和静息效应CD4(+) T细胞转移至未免疫的收养宿主后的迁移和持续存在。 Th1 和 Th2 效应细胞迁移至淋巴和非淋巴器官（腹膜、脂肪垫和肺）。相比之下，休息的效应细胞和初始细胞仅迁移到淋巴区域。粘附分子的表达，但不是趋化因子受体的表达，与进入非淋巴部位的能力相关。供体细胞在淋巴组织中比在非淋巴组织中存留的时间更长。当具有初始和记忆供体细胞的宿主受到抗原攻击时，效应器在原位发育，并且也迁移到非淋巴部位。记忆细胞显示出向非淋巴迁移的加速转变，符合这一点。与记忆效应器的形成。这些结果表明，只有最近激活的效应 T 细胞才能在没有抗原和炎症的情况下分散到非淋巴部位，并且当效应细胞恢复静止时，它们会失去这种能力。这些数据还表明，淋巴部位的记忆细胞寿命更长，并且与非淋巴部位的记忆细胞不平衡。
Recent studies suggest that effector T cells generated by immune responses migrate to multiple non-lymphoid sites, even those without apparent expression of antigen or inflammation. To investigate the ability of distinct CD4(+) T lymphocyte subsets to enter and persist in non-lymphoid, noninflamed compartments, we examined the migration and persistence of naive, effector, and rested effector CD4(+) T cells generated in vitro following transfer to nonimmunized adoptive hosts. Th1 and Th2 effectors migrated to both lymphoid and non-lymphoid organs (peritoneum, fat pads, and lung). In contrast, rested effectors and naive cells migrated only to lymphoid areas. Adhesion molecule expression, but not chemokine receptor expression, correlated with the ability to enter non-lymphoid sites. Donor cells persisted longer in lymphoid than in non-lymphoid sites. When hosts with naive and memory donor cells were challenged with antigen, effectors developed in situ, which also migrated to non-lymphoid sites. Memory cells showed an accelerated shift to non-lymphoid migration, in keeping. with memory effector formation. These results suggest that only recently activated effector T cells can disperse to non-lymphoid sites in the absence of antigen and inflammation, and as effectors return to rest, they lose this ability. These data also argue that memory cells in lymphoid sites are longer lived and not in equilibrium with those in non-lymphoid sites.