Plant Cytokinesis Is Orchestrated by the Sequential Action of the TRAPPII and Exocyst Tethering Complexes
Plant Cytokinesis Is Orchestrated by the Sequential Action of the TRAPPII and Exocyst Tethering Complexes
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DOI：
10.1016/j.devcel.2014.04.029
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发表时间：
2014-06-09
影响因子：
11.8
通讯作者：
Assaad, Farhah F.
中科院分区：
文献类型：
--
作者：
Rybak, Katarzyna;Steiner, Alexander;Assaad, Farhah F.
植物胞质分裂起始于一个短暂的膜室，即细胞板，并通过成熟过程完成，在此过程中细胞板变成横壁。从幼年期到成年期的转变是如何发生的，人们知之甚少。在这项研究中，我们监测拟南芥转运蛋白颗粒II（TRAPPII）和外囊栓系复合物在整个胞质分裂。我们发现，它们的外观主要是连续的，在胞质分裂的开始和结束时有短暂的重叠。TRAPPII复合物是细胞板生物发生所必需的，并且外囊是细胞板成熟所必需的。TRAPPII复合物在整个胞质分裂期间在细胞板处对质膜蛋白（包括胞外亚基）进行分类。我们表明，这两个拴系复合物物理相互作用，并提出他们的协调行动可能编排不仅植物，而且动物胞质分裂。
Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood. In this study, we monitor the Arabidopsis transport protein particle II (TRAPPII) and exocyst tethering complexes throughout cytokinesis. We show that their appearance is predominantly sequential, with brief overlap at the onset and end of cytokinesis. The TRAPPII complex is required for cell plate biogenesis, and the exocyst is required for cell plate maturation. The TRAPPII complex sorts plasma membrane proteins, including exocyst subunits, at the cell plate throughout cytokinesis. We show that the two tethering complexes physically interact and propose that their coordinated action may orchestrate not only plant but also animal cytokinesis.