CHARACTERIZATION OF A PIGMENT PRODUCED BY PSEUDOMONAS FLUORESCENS DURING 3-CHLOROBENZOATE CO-METABOLISM
CHARACTERIZATION OF A PIGMENT PRODUCED BY PSEUDOMONAS FLUORESCENS DURING 3-CHLOROBENZOATE CO-METABOLISM
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DOI：
10.1016/0045-6535(93)90014-v
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发表时间：
1993-09-01
期刊：
影响因子：
8.8
通讯作者：
MARCHETTI, L
中科院分区：
文献类型：
--
作者：
FAVA, F;DI GIOIA, D;MARCHETTI, L
发现一株荧光假单胞菌CP30能够通过3-氯儿茶酚和4-氯儿茶酚共同代谢3-氯苯甲酸。这两种中间体似乎与中间体的变暗有关。从CP30细胞和培养液中回收和纯化的黑色色素具有多酚类物质的许多化学和光谱特征。从化学降解研究中获得的分析数据和结果表明，这些卤化细菌色素的结构与迄今为止所描述的植物界的“儿茶酚-黑色素”密切相关。比较化学研究，包括细菌色素和体外制备的4-氯儿茶酚聚合物，支持CP30色素是“氯儿茶酚-黑色素”的假设。色素的产生似乎对CP30细胞形状的实质性改变负有责任。
A strain of Pseudomonas fluorescens, designated CP30, was found to be able to co-metabolize 3-chlorobenzoic acid via 3-chlorocatechol and 4-chlorocatechol. These two intermediates seem to be involved in the darkening of the medium. The black pigments produced, which were recovered and purified from the CP30 cells and culture broth, have a number of chemical and spectroscopic characteristics typical of polyphenols. The analytical data and the results obtained from chemical degradation studies suggest that the structure of these halogenated bacterial pigments is closely related to that of the ''catechol-melanins'' of the plant kingdom described until now.Comparative chemical studies, including both bacterial pigments and 4-chlorocatechol polymers prepared in vitro, supported the hypothesis that CP30 pigments are ''chlorocatechol-melanins''.The production of the pigment seems to be responsible for a substantial modification of the CP30 cell shape.