Endometrial IL-1β, IL-6 and TNF-α, mRNA expression in mares resistant or susceptible to post-breeding endometritis -: Effects of estrous cycle, artificial insemination and immunomodulation
Endometrial IL-1β, IL-6 and TNF-α, mRNA expression in mares resistant or susceptible to post-breeding endometritis -: Effects of estrous cycle, artificial insemination and immunomodulation
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DOI：
10.1016/s0165-2427(03)00137-5
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发表时间：
2003-11-15
影响因子：
1.8
通讯作者：
Bowden, RA
中科院分区：
文献类型：
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作者：
Fumuso, E;Gigure, S;Bowden, RA
在对持续性繁殖后子宫内膜炎 (PPBE) 耐药 (RM) 或易感 (SM) 的母马中，评估了促炎细胞因子白细胞介素 1β (IL-1β)、白细胞介素 6 (IL-6) 和肿瘤坏死因子 α (TNF-α) 的子宫内膜 mRNA 表达。根据繁殖记录和功能测试，从 2000 匹轻型杂交母马中选出 8 匹 RM 和 8 匹 SM。进行了三个实验来研究（i）基础条件下的转录模式； (ii) 人工授精 (AI) 后； (iii)在人工授精时施用免疫调节剂之后。在连续周期内进行子宫内膜活检：(i) 发情期，卵泡达到 35 毫米时和发情间期（排卵后 7 +/- 1 天）； (ii) AI后24小时，精液死亡（发情期）且处于发情间期； (iii)用草分枝杆菌细胞壁提取物(MCWE)制剂和All(含有死精液)处理后24℃，以及动情间期。通过实时PCR定量mRNA表达。在基础条件下，与 RM 相比，SM 在发情期的所有细胞因子以及在发情间期的 IL-1β 和 TNF-α 的 mRNA 表达均显着较高。 AI后，RM和SM发情情况无差异；然而，所有三种促炎细胞因子的 mRNA 表达均高于基础条件下。在发情间期，RM 的 IL-1β 和 TNF-α mRNA 表达显着低于 SM。当 AI 时施用 MCWE 时，未发现 RM 和 SM 的细胞因子诱导存在差异。总体而言，当表达较高时，所有三种细胞因子的 mRNA 表达相互之间具有良好的相关性。本研究表明，（i）在基础条件下，RM 的促炎细胞因子 mRNA 表达低于 SM，且对动情周期没有影响； (ii) AI 上调 RM 和 SM 中所有三种细胞因子的 mRNA 表达，并在后者的发情间期持续存在； (iii) AI时用MCWE治疗下调IL-1的mRNA表达，对SM有显着影响，其表现类似于RM。 MCWE 的免疫调节可能有助于恢复局部炎症机制的稳态，从而有助于预防母马繁殖后子宫内膜炎。 (C) 2003 Elsevier B.V. 保留所有权利。
Endometrial mRNA expression of the pro-inflammatory cytokines interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-alpha) was assessed in mares resistant (RM) or susceptible (SM) to persistent post-breeding endometritis (PPBE). Eight RM and eight SM, were selected based on reproductive records and functional tests out of a herd of 2000 light cross-type mares. Three experiments were done to study transcription patterns in (i) basal conditions; (ii) after artificial insemination (AI); and (iii) after administration of an immunomodulator at time of artificial insemination. Endometrial biopsies were taken during consecutive cycles: (i) at estrus, when follicles reached 35 mm and at diestrus (7 +/- 1 days after ovulation); (ii) at 24 h post-AI, with dead semen (estrus) and in diestrus; (iii) at 24 It after treatment with a Mycobacterium phlei cell-wall extract (MCWE) preparation and All (with dead semen), and at diestrus. mRNA expression was quantitated by real time PCR. Under basal conditions, SM had significantly higher mRNA expression of all cytokines in estrus and of IL-1beta and TNF-alpha in diestrus, compared to RM. After AI, there were no differences between RM and SM in estrus; however, mRNA expression for all three pro-inflammatory cytokines was higher than under basal conditions. In diestrus, RM showed significantly lower IL-1beta and TNF-alpha mRNA expression than SM. When MCWE was administered at time of AI, no differences between cytokine induction from RM and SM were found. Globally, mRNA expression for all three cytokines correlated well among themselves when expression was high. The present study showed that (i) in basal conditions RM had lower mRNA expression of pro-inflammatory cytokines than SM with no effect of estrous cycle; (ii) AI upregulated mRNA expression for all three cytokines in both RM and SM, with persistance in diestrus in the latter; (iii) treatment with MCWE at time of AI downregulated mRNA expression of IL-1 with significant effects in SM which behaved like RM. Immunomodulation with MCWE could be of help in restoring homeostatic local inflammatory mechanisms, thus assisting in the prophylaxis of post-breeding endometritis in mares. (C) 2003 Elsevier B.V. All rights reserved.