Efficient and scalable method in isolation of polymethoxyflavones from orange peel extract by supercritical fluid chromatography
Efficient and scalable method in isolation of polymethoxyflavones from orange peel extract by supercritical fluid chromatography
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DOI：
10.1016/j.jchromb.2006.09.010
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发表时间：
2007-02-01
影响因子：
3
通讯作者：
Ho, Chi-Tang
中科院分区：
文献类型：
--
作者：
Li, Shiming;Lambros, Ted;Ho, Chi-Tang
来自柑橘属的多甲氧基黄酮（PMFs）因其广泛的生物活性，如抗炎、抗癌和抗动脉粥样硬化特性而受到特别关注。近年来，对柑橘类水果中PMFs有益健康特性的探索急剧增加。然而，体内研究中纯PMFs的供应是一个限制因素，因为感兴趣的PMFs难以大规模分离。因此，开发一种高效、可扩展的PMFs分离方法是非常必要和重要的。本文报道了采用超临界色谱法（SFC）从甜橙（Citrus sinensis）果皮中高效、大规模分离出四种PMFs的新方法：褐皮素、橘子素、3,5,6,7,8,3',4'-七甲基甲黄酮和5,6,7,4'-四甲基甲黄酮。(c) 2006 Elsevier B.V.版权所有
Polymethoxyflavones (PMFs) from citrus genus are of particular interest because of their broad spectrum of biological activities, such as anti-inflammatory, anti-carcinogenic, and anti-atherogenic properties. Recently, the exploration into the beneficial health properties of PMFs in citrus fruits has dramatically increased. However, the supply of pure PMFs in the in vivo study is a limiting factor due to the difficulties in large-scale isolation of the interested PMFs. Therefore, the development of an efficient and a scalable separation method of PMFs is necessary and significant. In this paper, we report a newly developed method for efficient and relatively large-scale isolation of four PMFs from sweet orange (Citrus sinensis) peel by employing supercritical chromatography (SFC): nobiletin, tangeretin, 3,5,6,7,8,3',4'-heptamethoxyflavone and 5,6,7,4'-tetramethoxyflavone. (c) 2006 Elsevier B.V. All rights reserved.