Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury
Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury
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DOI：
10.1016/j.jcin.2009.05.014
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发表时间：
2009-08-01
影响因子：
11.3
通讯作者：
Lythgoe, Mark F.
中科院分区：
文献类型：
--
作者：
Kyrtatos, Panagiotis G.;Lehtolainen, Pauliina;Lythgoe, Mark F.
目的利用体外磁化装置对血管内皮祖细胞进行磁性标记，使其定位于动脉损伤部位。背景：循环内皮祖细胞参与血管再内皮化和缺血后新生血管等生理过程。然而，细胞治疗的成功与否取决于能否将细胞输送到损伤部位。流式细胞仪检测细胞存活率和分化程度。在有限元模拟和体外血流测试的基础上，对大鼠颈总动脉进行血管成形术，剥离血管内皮细胞，并在有无外磁装置的情况下给予内皮祖细胞12min。结果计算机模拟表明，从与大鼠颈总动脉相似的血管中成功地进行了外磁细胞靶向；相应地，在体外流动系统中，细胞捕获增加了6倍。体内植入24小时后，靶向性增强5倍的细胞在损伤部位的滞留。结论使用外加磁性装置，我们能够增强EPC在颈总动脉损伤部位的定位。这项技术可以更广泛地适用于定位其他器官的细胞，并可能为全身注射细胞疗法提供有用的工具。(J Am Coll心脏ol Intv 2009；2：794-802)(C)美国心脏病学会基金会2009年
Objectives We sought to magnetically tag endothelial progenitor cells (EPCs) with a clinical agent and target them to a site of arterial injury using a magnetic device positioned outside the body.Background Circulating EPCs are involved in physiological processes such as vascular re-endothelialization and post-ischemic neovascularization. However, the success of cell therapies depends on the ability to deliver the cells to the site of injury.Methods Human EPCs were labeled with iron oxide superparamagnetic nanoparticles. Cell viability and differentiation were tested using flow cytometry. Following finite element modeling computer simulations and flow testing in vitro, angioplasty was performed on rat common carotid arteries to denude the endothelium and EPCs were administered with and without the presence of an external magnetic device for 12 min.Results Computer simulations indicated successful external magnetic cell targeting from a vessel with flow rate similar to a rat common carotid artery; correspondingly there was a 6-fold increase in cell capture in an in vitro flow system. Targeting enhanced cell retention at the site of injury by 5-fold at 24 h after implantation in vivo.Conclusions Using an externally applied magnetic device, we have been able to enhance EPC localization at a site of common carotid artery injury. This technology could be more widely adapted to localize cells in other organs and may provide a useful tool for the systemic injection of cell therapies. (J Am Coll Cardiol Intv 2009;2:794-802) (C) 2009 by the American College of Cardiology Foundation